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© 1988 Oxford University Press

research-article

Comparison of Paraquat-Specific Murine Monoclonal Antibodies Produce by in Vitro and in Vivo Immunization1

SHANE C. JOHNSTON, MARK BOWLES, DONALD J. WINZOR* and SUSAN M. POND2

Department of Medicine, University of Queensland. Princess Alexandra Hospital Brisbane, Queensland, 4102, Australia. *Department of Biochemistry, University of Queensland St. Lucia, Queensland, 4067, Australia.

Received October 13, 1987; accepted March 14, 1988

Comparison of Paraquat-Specific Murine Monoclonal Antibodies Produced by in Vitro and in Vitro Immunization. Johnston, S. C, Bowles, M., Winzor, D. J., and Pond, S. M. (1988). Fundam Appl. Toxicol. 11,261 –267. The techniques of in vitro and in vivo immunization have been used to produce murine hybridomas secreting anti-paraquat IgM and igG antibodies, respectively. The monoclonal antibodies from these IgM-secreting and IgG-secreting cell lines have been purified by HPLC and characterized by ELISA for their affinities to eliciting antigen and cross-reactivity. Both antibody preparations exhibited high selectivity and similar affinities for paraquat. There was only a minor degree of interaction with a range of paraquat analogs that differed in the nature and position of group substituents.


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