Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by RAFELGHEM, M. J.V.
Right arrow Articles by ANDERSON, M. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by RAFELGHEM, M. J.V.
Right arrow Articles by ANDERSON, M. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1988 Oxford University Press

research-article

The Effects of Perfluorodecanoic Acid on Hepatic Stearoyl-Coenzyme A 1 Desaturase and Mixed Function Oxidase Activities in Rats

MARC J.VAN RAFELGHEM1,* and MELVIN E. ANDERSON{dagger}

*Department of Pharmacology and Toxicology, Wright Stale University Dayton, Ohio 45435 {dagger}Armstrong Aerospace Medical Research Laboratory, Toxic Hazards Division, Biochemical Branch, Wright-Patterson Air Force Base Ohio 45433

Received January 27, 1988; accepted May 25, 1988

The Effects of Perfluorodecanoic Acid on Hepatic Stearoyl-Coenzyme A Desaturase and Mixed Function Oxidase Activities in Rats.VAN RAFELGHAM, M. J., AND ANDERSON, M. E. (1988). Fundam. Appl. Toxicol. 11, 503-510. Perfluorodecanoic acid (PFDA) causes a dioxin-lilce toxic syndrome and alters the hepatic oleate/stearate ratio in rats. The acute toxic effects of a single ip dose (50 mg/kg) of PFDA on hepatic stearoyl-CoA desaturase and mixed function oxidases were studied in male Fischer-344 rats, 14 days after dosing. PFDA causes a marked decrease in food intake in rats, resulting in severe body weight loss with delayed lethality (2-3 weeks after dosing). To distinguish the effects of hypophagia from those caused by PFDA, pair-fed control rats were used in addition to ad libitum-fed controls. Stearoyl-CoA desaturase activity, responsible for the conversion of stearoyl-CoA to oleoyl-CoA, was absent in both PFDA dosed rats and their pair-fed controls at Day 14. Electron transfer through the desaturase system was significantly reduced in PFDA-treated rats only, and in these rats there was a significant reduction in microsomal cytochrome an important component of this electron transfer system. Pentobarbital sleeping times were significantly prolonged in both the PFDA-dosed and pairfed rats, as compared with the ad libitumfed controls. This effect was more pronounced in PFDA-dosed rats. Waking plasma pentobarbital concentration was similar in all treatment groups. Hepatic microsomal cytochrome PASO content was unaffected. Aminopyrine N-de-methylase activity was greatly reduced in PFDA-dosed rats. Although pairfed controls also had reduced demethylase activity, it was not as pronounced as in PFDA-dosed rats, and was probably due to the fasted condition of these animals. Although the mechanism of action of PFDA is not known, it is possible that PFDA affects microsomal enzymes by altering the structure and/or function of the membranes in which they are located, through effects on lipid metabolism.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.