Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by WALLIG, M. A
Right arrow Articles by WILLHITE, C. C
Right arrow Search for Related Content
PubMed
Right arrow Articles by WALLIG, M. A
Right arrow Articles by WILLHITE, C. C
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1989 Oxford University Press

research-article

The Relationship of Vehicle to Target Organ Toxicology Induced by the Naturally Occurring Nitrile 1-Cyano-2-hydroxy-3-butene

MATTHEW A WALLIG*,1, DANIEL H GOULD*, JAN VAN STEENHOUSE*, MARTIN J FETTMAN* and CALVIN C WILLHITE{dagger}

*Department of Pathology, Colorado State University Fort Collins, Colorado 80523 {dagger}California Department of Health Services Berkeley, California 94704

Received October 26, 1987; accepted August 1, 1988

The Relationship of Vehicle to Target Organ Toxicology Induced by the Naturally Occurring Nitrile 1-Cyano-2-hydroxy-3-butene. WALLIG, M. A., GOULD, D. H., VAN STEENHOUSE, J., FETTMAN, M. J., AND WILLHITE, C. C. (1989). Fundam. Appl. Toxicol 12, 377–385. The effects of gavage vehicle on the acute toxicity of the naturally occurring nitrile 1-cyano-2-hy-droxy-3-butene (CHB) were investigated by oral administration of 200 mg/kg body wt/day CHB to male CDF (F-344/Crl BR) rats for 2 days. The vehicles studied here were distilled water, 5% aqueous Tween 20, and corn oil. Liver, kidney, and pancreas were examined histologically and the differences in lesion incidence and severity were assessed. The effects of gavage vehicle on nitrile-induced elevations of daily urinary thiocyanate excretion and tissue glutathione concentrations were also assessed. The pancreatotoxicity of CHB was present regardless of vehicle and consisted of apoptosis of pancreatic acinar cells, infiltration of pancreatic lobules by macrophages, and acinar atrophy and disorganization. CHB in water alone was associated with the least pancreatotoxic effect, whereas the aqueous Tween vehicle was associated with more severe CHB-induced pancreatic lesions. CHB-induced elevations of tissue nonprotein thiol and gluta thione concentrations occurred in all treatment groups, but the values were-elevated significantly less in the pancreata of CHB/Tween-treated rats than in those of rats given CHB in water or corn oil. By contrast, the greatest elevation in daily urinary thiocyanate excretion occurred in rats given CHB in aqueous Tween, indicating increased biotransformation of CHB to cyanide when Tween 20 was used as a vehicle. These results illustrate the difficulty of identifying suitable vehicles for administration of lipophilic compounds in toxicology studies.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.