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© 1991 Oxford University Press

research-article

Phagolysosomal pH and Location of Particles in Alveolar Macrophages

KRISTINA NYBERG*, UNO JOHANSSON{dagger}, ANNE JOHANSSON*,{ddagger} and PER CAMNER*

*Section of Lung Medicine, Unit of Environmental Hygiene, Institute of Environmental Medicine, Karolinska Institute S-104 01 Stockholm, Sweden {dagger}Department of Pathology II, University of Linköping S-581 85 Linköping, Sweden {ddagger}The Wenner-Gren Institute, the Arrhenius Laboratories for Natural Sciences, University of Stockholm S-106 91 Stockholm, Sweden

Received April 16, 1990; accepted November 28, 1990

Phagolysosomal pH and Location of Particles in Alveolar Macrophages. NYBERG, K., JOHANSSON, U., JOHANSSON, A., AND CAMNER, P. (1991). Fundam. Appl. Toxicol. 16, 393–400. Fluorescein-labeled silica particles (FSP) were instilled into the tracheae of rabbits. Groups of four rabbits were killed after 24 hr, 1 week, 1 month, or 3 months and their lungs were lavaged. Phagolysosomal pH in the alveolar macrophages (AM) was measured using microscope fluorometry with FSP as a probe. Due to the marked decline of the fluorescence intensities from the FSP between 1 and 3 months after instillation, it was not possible to measure pH at 3 months, but the values from 24 hr, 1 week, and 1 month were quite similar, with group means of 4.8 and 4.9, respectively. Phagolysosomal pH in AM which phagocytized the FSP in vitro showed values about half a pH unit higher. AM from rabbits lavaged at 1 week had more lysosomes in contact with the FSP-containing phagolysosomes and a higher degree of vacuolization between the FSP and the phagolysosomal membrane than AM lavaged at 1 day. The location of the FSP in the AM appeared to be similar in rabbits lavaged after 1 week and 3 months. In histologic sections from the lungs the large majority of the FSP were within cells at all time points.


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