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© 1982 Oxford University Press

research-article

Mutagenicity of Diesel Exhaust Particle Extracts: Influence of Driving Cycle and Environmental Temperature

C.R. CLARKA, J.S. DUTCHERA, A.L. BROOKSA, R.O. McCLELLANA, W.F. MARSHALLB and T.M. NAMANB

AInhalation Toxicology Research Institute P.O. Box 5890, Albuquerque, New Mexico 87185 BBartlesville Energy Technology Center Bartlesville, Oklahoma 74003

Mutagenicity of Diesel Exhaust Particle Extracts: Influence of Driving Cycle and Environmental Temperature. Clark, C.R., Dutcher, J.S., Brooks, A.L., McClellan, R.O., Marshall, W.F., and Naman, T.M. (1982). Fundam. Appl. Toxicol. 2:153-157. General Motors and Volkswagen diesel passenger cars (1980 and 1981 model year) were operated on a climate controlled chassis dynomometer and the particulate portion of the exhaust was collected on high volume filters. Dichloromethane extracts of the exhaust particles (soot) collected while the cars were operated under simulated highway, urban and congested urban driving cycles were assayed for mutagenicity in Salmonella strains TA-98 and TA-100. Driving pattern did not significantly influence the mutagenic potency of the exhaust particle extracts or estimates of the amount of mutagenicity emitted from the exhaust despite large differences in particle emission rates and extractable fraction of the particles. Mutagenicity of extracts of exhaust particles collected while the vehicles were operated at test chamber temperatures of 25, 50, 75 and 100°F were also very similar. The results suggest that driving pattern and environmental temperature do not significantly alter the emission of genotoxic combustion products from the exhaust.


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