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© 1998 Oxford University Press

research-article

Assessing the Predictiveness of the Syrian Hamster Embryo Cell Transformation Assay for Determining the Rodent Carcinogenic Potential of Single Ring Aromatic/Nitroaromatic Amine Compounds

Gary A. Kerckaert, Robert A. LeBoeuf and Robert J. Isfort

Corporate Professional & Regulatory Services, The Procter & Gamble Company, Miami Valley Laboratories P.O. Box 538707, Cincinnati, Ohio 45253-8707

Received April 7, 1997; accepted June 27, 1997

The pH 6.7 Syrian hamster embryo (SHE) cell transformation assay was used to test the morphological transformation potential of 5 rodent carcinogenic single ring aromatic/nitroaromatic amine compounds: 2-amino-4-nitrotoluene, 2,4-diaminotoluene, 2,4-dinitrotoluene, o-anisidine hydrochloride, and o-toluidine; and 5 noncarcinogenic single ring aromatic/nitroaromatic amine compounds: 2,6-diaminotoluene, 2,4-dimethoxyaniline hydrochloride, 4-nitro-o-phenylenediamine, p-phenylenediamine dihydrochloride, and HC Blue No. 2. All 5 rodent carcinogens produced significant morphological transformation in a dose-responsive manner. None of the 5 noncarcinogens yielded significant transformation at any of the doses tested. Therefore, the concordance between the pH 6.7 SHE cell transformation assay and rodent carcinogenicity for these 10 single ring aromatic/nitroaromatic amine compounds is 100%. In contrast, the concordance between the standard SHE cell transformation assay and rodent carcinogenicity for 13 single ring aromatic/nitroaromatic amine compounds was 62%. For 5 aromatic/nitroaromatic amine compounds which were tested in both standard and pH 6.7 SHE cell transformation assays (i.e., a subset of the above two databases), the concordance between the standard SHE cell transformation assay and the rodent bioassay was 40%, while the concordance between the pH 6.7 SHE cell transformation assay and the rodent bioassay was 100%. This relatively high concordance between the pH 6.7 SHE cell transformation assay and rodent bioassay results demonstrates the utility of the pH 6.7 SHE cell transformation assay for predicting the rodent carcinogenic potential of single ring aromatic/nitroaromatic amine compounds.


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This article has been cited by other articles:


Home page
 Toxicol PatholHome page
Syrian Hamster Embryo Assay Working Group, R.J. Mauthe, D.P. Gibson, R.T. Bunch, and L. Custer
Response to "Alternative Models for Carcinogenicity Testing: Weight of Evidence Across Models" Sam Cohen, Toxicologic Pathology (2001) 29(Suppl.): 183--190
Toxicol Pathol, February 1, 2002; 30(2): 292 - 293.
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