Toxicological Sciences, Vol 47, 158-169, Copyright © 1999 by Society of Toxicology
D Desaulniers, K Leingartner, M Wade, E Fintelman, A Yagminas and WG Foster
3,3'4,4',5-Pentachlorobiphenyl (PCB 126) and 2,2',4,4',5,5'-
hexachlorobiphenyl (PCB 153) were administered to adult male rats in order
to identify sensitive indicators of endocrine disruption. We tested the
hypothesis that PCB exposure modifies follicle-stimulating hormone (FSH)
pituitary isoforms, as well as the pituitary and serum concentrations of
FSH, luteinizing hormone (LH), growth hormone, prolactin, and
thyroid-stimulating hormone (TSH). Effects on serum levels of thyroxine
(T4) and testosterone (T), and prostate androgen receptor content, were
also tested. In one experiment, 5 groups of 8 rats each received two i.p.
injections, one day apart, of either corn oil or 6.25, 25, 100 or 400
micrograms/kg/day of PCB 126. Decreases (p < 0.05) in the serum
concentrations of T4 and LH started at doses of 25 and 100
micrograms/kg/day, respectively. Serum FSH concentrations were reduced (p =
0.07) in the highest dose group. In contrast, pituitary content of FSH and
LH increased with PCB-126 doses (p = 0.004, p = 0.002, respectively).
Despite changes in reproductive hormones, PCB-126 had no effect on the
androgen receptor content of the prostate. The effect of PCB-126 was tested
in the hemicastrated rat, and suggested adverse effects on testosterone
secretion. To test the effects of PCB exposure on FSH pituitary isoforms, 4
groups of 10 male rats received two i.p. injections, one day apart, of
either corn oil, PCB 153 (25 mg/kg/day), estradiol-17 beta (E2; 20
micrograms/kg/day), or PCB 126 (0.1 mg/kg/day). Serum T4 levels were higher
(p < 0.01) in the E2 and PCB 153 groups, and slightly reduced in the PCB
126-treated groups, compared to controls. Simultaneous purification of
pituitary FSH and TSH isoforms was performed by HPLC, using two
chromatofocusing columns in series. In contrast to TSH isoforms, the
distribution of FSH isoforms over the chromatography run differed slightly
between treatment groups; the amounts of FSH isoform eluted during the pH
gradient were lower (p < 0.05) in E2 and PCB 153-treated rats than in
control or PCB 126-treated rats. The similarity between the effects of E2
and PCB 153 on T4 and FSH isoforms supports the contention that PCB 153
possesses estrogenic properties. Serum LH and T4 concentrations were the
most sensitive and practical endocrine indicators of PCBs 126 and 153
exposure in male rats.
ARTICLES
Effects of acute exposure to PCBs 126 and 153 on anterior pituitary and thyroid hormones and FSH isoforms in adult Sprague Dawley male rats
Environmental and Occupational Toxicology Division, Department of Health, Ottawa, Ontario, Canada. Daniel_Desaulniers@hc-sc.ca
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Hedge, M. J. DeVito, and K. M. Crofton In Vivo Acute Exposure to Polychlorinated Biphenyls: Effects on Free and Total Thyroxine in Rats International Journal of Toxicology, September 1, 2009; 28(5): 382 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Fisher, J. Campbell, S. Muralidhara, J. V. Bruckner, D. Ferguson, M. Mumtaz, B. Harmon, J. M. Hedge, K. M. Crofton, H. Kim, et al. Effect of PCB 126 on Hepatic Metabolism of Thyroxine and Perturbations in the Hypothalamic-Pituitary-Thyroid Axis in the Rat Toxicol. Sci., March 1, 2006; 90(1): 87 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C Oskam, J. L Lyche, A. Krogenaes, R. Thomassen, J. U Skaare, R. Wiger, E. Dahl, T. Sweeney, A. Stien, and E. Ropstad Effects of long-term maternal exposure to low doses of PCB126 and PCB153 on the reproductive system and related hormones of young male goats Reproduction, November 1, 2005; 130(5): 731 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Desaulniers, G. M. Cooke, K. Leingartner, K. Soumano, J. Cole, J. Yang, M. Wade, and A. Yagminas Effects of Postnatal Exposure to a Mixture of Polychlorinated Biphenyls, p,p'-dichlorodiphenyltrichloroethane, and p-p'-dichlorodiphenyldichloroethene in Prepubertal and Adult Female Sprague-Dawley Rats International Journal of Toxicology, March 1, 2005; 24(2): 111 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yamamoto, A. Narita, M. Kagohata, M. Shirai, F. Akahori, and K. Arishima Effects of Maternal Exposure to 3,3',4,4',5-Pentachlorobiphenyl (PCB126) or 3,3',4,4',5,5'-Hexachlorobiphenyl (PCB169) on Testicular Steroidogenesis and Spermatogenesis in Male Offspring Rats J Androl, March 1, 2005; 26(2): 205 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Muscat, J. A. Britton, M. V. Djordjevic, M. L. Citron, M. Kemeny, E. Busch-Devereaux, B. Pittman, and S. D. Stellman Adipose Concentrations of Organochlorine Compounds and Breast Cancer Recurrence in Long Island, New York Cancer Epidemiol. Biomarkers Prev., December 1, 2003; 12(12): 1474 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Desaulniers, K. Leingartner, B. Musicki, A. Yagminas, G.-H. Xiao, J. Cole, L. Marro, M. Charbonneau, and B. K. Tsang Effects of Postnatal Exposure to Mixtures of Non-ortho-PCBs, PCDDs, and PCDFs in Prepubertal Female Rats Toxicol. Sci., October 1, 2003; 75(2): 468 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Craft, M. J. DeVito, and K. M. Crofton Comparative Responsiveness of Hypothyroxinemia and Hepatic Enzyme Induction in Long-Evans Rats Versus C57BL/6J Mice Exposed to TCDD-like and Phenobarbital-like Polychlorinated Biphenyl Congeners Toxicol. Sci., August 1, 2002; 68(2): 372 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Khan and P. Thomas Disruption of Neuroendocrine Control of Luteinizing Hormone Secretion by Aroclor 1254 Involves Inhibition of Hypothalamic Tryptophan Hydroxylase Activity Biol Reprod, March 1, 2001; 64(3): 955 - 964. [Abstract] [Full Text]
|
|
|
|
|
|
S. D. Stellman, M. V. Djordjevic, J. A. Britton, J. E. Muscat, M. L. Citron, M. Kemeny, E. Busch, and L. Gong Breast Cancer Risk in Relation to Adipose Concentrations of Organochlorine Pesticides and Polychlorinated Biphenyls in Long Island, New York Cancer Epidemiol. Biomarkers Prev., November 1, 2000; 9(11): 1241 - 1249. [Abstract] [Full Text]
|
|