Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Woolhiser, M. R.
Right arrow Articles by Meade, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woolhiser, M. R.
Right arrow Articles by Meade, B. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Toxicological Sciences 55, 343-351 (2000)
Copyright © 2000 by the Society of Toxicology

Immunological Responses of Mice following Administration of Natural Rubber Latex Proteins by Different Routes of Exposure

Michael R. Woolhiser*,{dagger}, Albert E. Munson* and B. Jean Meade*,1

* National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505; and {dagger} Department of Pharmacology & Toxicology, Medical College of Virginia of Virginia Commonwealth University, Richmond, Virginia 23298

Although the prevalence of IgE-mediated latex allergy has increased over the past decade, the circumstances which culminate in sensitization remain uncertain. The objective of these studies was to evaluate the role which sensitization route plays in the development of latex allergy using murine models representative of potential exposure routes by which health care workers (topical and respiratory) and spina bifida patients (subcutaneous) may be sensitized. BALB/c mice administered latex proteins by the subcutaneous, topical, intranasal, or intratracheal routes exhibited dose-responsive elevations in total IgE. In vitro splenocyte stimulation initially demonstrated specificity of the murine immune response to latex proteins. Subsequently, immunoblot analysis was used to compare latex-specific IgE production amongst sensitization routes. Immunoblots of IgE from subcutaneously sensitized mice demonstrated recognition of latex proteins with molecular weights near 14 kDa and 27 kDa. These protein sizes are consistent with the molecular weights of major latex allergens (Hev b 1 and Hev b 3), to which high percentages of spina bifida patients develop antibodies. Mice sensitized by intratracheal or topical administration exhibited combined IgE recognition of latex proteins near 14 kDa, 35 kDa, and 92 kDa. These molecular weights are similar to other latex allergens (Hev b 6, Hev b 2, and Hev b 4) commonly recognized by IgE of health care workers. Mice sensitized to latex proteins by topical, intranasal, or intratracheal exposures exhibited bronchoconstriction as evaluated by whole body plethysmography following respiratory challenge with latex proteins. Subcutaneously sensitized mice were unresponsive. These differences in latex-specific IgE immunoblot profiles and altered pulmonary function amongst the four different sensitization routes suggest that exposure routes leading to sensitization may play a role in determining the primary allergen(s), and the clinical manifestation of the allergic responses.

Key Words: mouse; latex; allergy; IgE; topical; respiratory; plethysmography; NRL; Hev B; PENH; bronchoconstriction.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Journal of the American Dental AssociationHome page
C. P. HAMANN, L. G. DEPAOLA, and P. A. RODGERS
Occupation-related allergies in dentistry
J Am Dent Assoc, April 1, 2005; 136(4): 500 - 510.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
A. K. Farraj, J. R. Harkema, T.-R. Jan, and N. E. Kaminski
Immune Responses in the Lung and Local Lymph Node of A/J Mice to Intranasal Sensitization and Challenge with Adjuvant-Free Ovalbumin
Toxicol Pathol, June 1, 2003; 31(4): 432 - 447.
[Abstract] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. L. Hardy, L. Kenins, A. C. Drew, J. M. Rolland, and R. E. O'Hehir
Characterization of a Mouse Model of Allergy to a Major Occupational Latex Glove Allergen Hev b 5
Am. J. Respir. Crit. Care Med., May 15, 2003; 167(10): 1393 - 1399.
[Abstract] [Full Text] [PDF]

Home page
 ANN OCCUP HYGHome page
D. HEEDERIK, P. S. THORNE, and G. DOEKES
Health-based Occupational Exposure Limits for High Molecular Weight Sensitizers: How Long is the Road We Must Travel?
Ann. Hyg., July 1, 2002; 46(5): 439 - 446.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
R. Brehler and B. Kutting
Natural Rubber Latex Allergy: A Problem of Interdisciplinary Concern in Medicine
Arch Intern Med, April 23, 2001; 161(8): 1057 - 1064.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. Toraason, G. Sussman, R. Biagini, J. Meade, D. Beezhold, and D. Germolec
Latex Allergy in the Workplace
Toxicol. Sci., November 1, 2000; 58(1): 5 - 14.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
B. B. Hayes, A. Afshari, L. Millecchia, P. A. Willard, S. P. Povoski, and B. J. Meade
Evaluation of Percutaneous Penetration of Natural Rubber Latex Proteins
Toxicol. Sci., August 1, 2000; 56(2): 262 - 270.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.