Toxicological Sciences 56, 240-249 (2000)
Copyright © 2000 by the Society of Toxicology
Purified Fumonisin B1 Decreases Cardiovascular Function but Does Not Alter Pulmonary Capillary Permeability in Swine
,1
,2
* Departments of Veterinary Clinical Medicine,
Veterinary Pathobiology, and
§ Veterinary Biosciences, College of Veterinary Medicine, University of Illinois, Urbana, Illinois 61802; and
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, Maryland 20708
Fumonisins are mycotoxins produced by Fusarium verticillioides, which induce acute pulmonary edema in swine. We previously reported that ingestion of fumonisin-containing culture material decreases cardiovascular function in swine (1996,a,b; Fundam. Appl. Toxicol. 31, 169–172; 33, 140–148; 1999, Am. J Vet. Res. 60, 1291–1300). The main purpose of this study was to confirm that fumonisin B1 was responsible for the observed cardiovascular changes. Treated pigs (n = 6) were given daily intravenous injections of purified fumonisin B1 at 1 mg/kg for 4 days, while controls (n = 6) were injected with equal volumes of saline. On day 5, pigs were anesthetized with butorphanol-chloralose and instrumented for hemodynamic studies. Terminally, bronchoalveolar lavage was performed on each pig to determine the relative permeability index of the pulmonary endothelium. Fumonisin B1-treated pigs had marked decreases in the maximal rate of change of left ventricular pressure (dP/dtmax), mean aortic pressure, cardiac output, and arterial pO2, accompanied by increases in mean pulmonary artery pressure, oxygen extraction ratio, and blood hemoglobin concentration. Plasma and left ventricular sphingosine and sphinganine concentrations were markedly increased in treated pigs at day 5; however, there was no difference in the relative permeability index between groups. Serum cholesterol concentrations and activities of hepatic-derived enzymes were increased, and hepatocyte apoptosis and mitoses were present in the livers of fumonisin-treated pigs. In the lungs of treated pigs, there was proteinaceous edema and membranous accumulations in capillary endothelial cells. These results indicate that cardiovascular function is altered by fumonisin B1, and that fumonisin-induced pulmonary edema is caused by left-sided heart failure and not by altered endothelial permeability. Because of the potential for contamination of human foodstuffs by fumonisins, the cardiovascular toxicity of these compounds must be taken into consideration.
Key Words: fumonisin; cardiovascular; heart failure; pulmonary edema; swine.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H Stockmann-Juvala and K Savolainen A review of the toxic effects and mechanisms of action of fumonisin B1 Human and Experimental Toxicology, November 1, 2008; 27(11): 799 - 809. [Abstract] [PDF]
|
|
|
|
 |
|
 S.-H. Hsiao, M. E. Tumbleson, P. D. Constable, and W. M. Haschek Use of formalin-fixed tissues to determine fumonisin B1-induced sphingolipid alterations in swine J Vet Diagn Invest, July 1, 2007; 19(4): 425 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Voss, J. Liu, S. P. Anderson, C. Dunn, J. D. Miller, J. R. Owen, R. T. Riley, C. W. Bacon, and J. C. Corton Toxic Effects of Fumonisin in Mouse Liver Are Independent of the Peroxisome Proliferator-Activated Receptor {alpha} Toxicol. Sci., January 1, 2006; 89(1): 108 - 119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Piva, G. Casadei, G. Pagliuca, E. Cabassi, F. Galvano, M. Solfrizzo, R. T. Riley, and D. E. Diaz Activated carbon does not prevent the toxicity of culture material containing fumonisin B1 when fed to weanling piglets J Anim Sci, August 1, 2005; 83(8): 1939 - 1947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Hsiao, P. D. Constable, G. W. Smith, and W. M. Haschek Effects of Exogenous Sphinganine, Sphingosine, and Sphingosine-1-Phosphate on Relaxation and Contraction of Porcine Thoracic Aortic and Pulmonary Arterial Rings Toxicol. Sci., July 1, 2005; 86(1): 194 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. Constable, G. W. Smith, G. E. Rottinghaus, M. E. Tumbleson, and W. M. Haschek Fumonisin-induced blockade of ceramide synthase in sphingolipid biosynthetic pathway alters aortic input impedance spectrum of pigs Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2034 - H2044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. P. Dragan, W. R. Bidlack, S. M. Cohen, T. L. Goldsworthy, G. C. Hard, P. C. Howard, R. T. Riley, and K. A. Voss Implications of Apoptosis for Toxicity, Carcinogenicity, and Risk Assessment: Fumonisin B1 as an Example Toxicol. Sci., May 1, 2001; 61(1): 6 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mathur, P. D. Constable, R. M. Eppley, M. E. Tumbleson, G. W. Smith, W. J. Tranquilli, D. E. Morin, and W. M. Haschek Fumonisin B1 Increases Serum Sphinganine Concentration but Does Not Alter Serum Sphingosine Concentration or Induce Cardiovascular Changes in Milk-Fed Calves Toxicol. Sci., April 1, 2001; 60(2): 379 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mathur, P. D. Constable, R. M. Eppley, A. L. Waggoner, M. E. Tumbleson, and W. M. Haschek Fumonisin B1 Is Hepatotoxic and Nephrotoxic in Milk-Fed Calves Toxicol. Sci., April 1, 2001; 60(2): 385 - 396. [Abstract] [Full Text] [PDF]
|
|