Toxicological Sciences 65, 192-199 (2002)
Copyright © 2002 by the Society of Toxicology
ENVIRONMENTAL TOXICOLOGY |
2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxicity in the Zebrafish Embryo: Altered Regional Blood Flow and Impaired Lower Jaw Development
* Department of Toxicology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan;
Veterinary Internal Medicine II, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan;
Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan; and
School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53705–2222
The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure on regional red blood cell (RBC) perfusion rate, as an index of blood flow, and lower jaw development were investigated quantitatively in zebrafish embryos (Danio rerio) during early development. As revealed by observation of live embryos and alcian-blue staining, TCDD retarded lower jaw development in a concentration-dependent manner with only a minor inhibitory effect on total body length. Both inhibitory effects were significant as early as 60 h postfertilization (hpf), at which time the area of goosecoid (gsc) mRNA expression was clearly reduced in the lower jaw. To examine effects of TCDD on RBC perfusion rate, time-lapse recording was performed using a digital video camera attached to a light microscope. TCDD did not show marked effects on RBC perfusion rate until 72 hpf, when vessel-specific effects emerged. TCDD severely inhibited RBC perfusion rate in intersegmental arteries of the trunk, but only modestly and slightly inhibited RBC perfusion rate in certain vessels of the head such as the central arteries and optic vein. Conversely, at both 72 and 84 hpf, TCDD significantly increased RBC perfusion rate in the hypobranchial artery branching to the lower jaw primordia, and then reduced it at 96 hpf. RBC perfusion rate in all vessels examined in TCDD-exposed embryos was inhibited at 96 hpf. The zebrafish aryl hydrocarbon receptor 2 (zfAhR2) mRNA was strongly expressed in the lower jaw primordia at 48 hpf, and expression of this transcript was augmented by TCDD treatment. Thus, TCDD exposure of the zebrafish embryo has a disruptive effect on local circulation and lower jaw cartilage growth. Initially, TCDD may act directly on the lower jaw primordia to impair lower jaw development. Reductions in hypobranchial RBC perfusion rate occurred well after the initial retardation in lower jaw development had become apparent, and may contribute further to the effect.
Key Words: Ah receptor (AhR); 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD); zebrafish; embryo; developmental toxicity; craniofacial; jaw; cardiovascular; blood flow; cartilage.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H.-C. Yin, H.-P. Tseng, H.-Y. Chung, C.-Y. Ko, W.-S. Tzou, D. R. Buhler, and C.-H. Hu Influence of TCDD on Zebrafish CYP1B1 Transcription during Development Toxicol. Sci., May 1, 2008; 103(1): 158 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Antkiewicz, R. E. Peterson, and W. Heideman Blocking Expression of AHR2 and ARNT1 in Zebrafish Larvae Protects Against Cardiac Toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxicol. Sci., November 1, 2006; 94(1): 175 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Carney, J. Chen, C. G. Burns, K. M. Xiong, R. E. Peterson, and W. Heideman Aryl Hydrocarbon Receptor Activation Produces Heart-Specific Transcriptional and Toxic Responses in Developing Zebrafish Mol. Pharmacol., August 1, 2006; 70(2): 549 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Andreasen, L. K. Mathew, and R. L. Tanguay Regenerative Growth Is Impacted by TCDD: Gene Expression Analysis Reveals Extracellular Matrix Modulation Toxicol. Sci., July 1, 2006; 92(1): 254 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Prasch, R. L. Tanguay, V. Mehta, W. Heideman, and R. E. Peterson Identification of Zebrafish ARNT1 Homologs: 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxicity in the Developing Zebrafish Requires ARNT1 Mol. Pharmacol., March 1, 2006; 69(3): 776 - 787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Lavine, A. J. Rowatt, T. Klimova, A. J. Whitington, E. Dengler, C. Beck, and W. H. Powell Aryl Hydrocarbon Receptors in the Frog Xenopus laevis: Two AhR1 Paralogs Exhibit Low Affinity for 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) Toxicol. Sci., November 1, 2005; 88(1): 60 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Hill, H. Teraoka, W. Heideman, and R. E. Peterson Zebrafish as a Model Vertebrate for Investigating Chemical Toxicity Toxicol. Sci., July 1, 2005; 86(1): 6 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. Handley-Goldstone, M. W. Grow, and J. J. Stegeman Cardiovascular Gene Expression Profiles of Dioxin Exposure in Zebrafish Embryos Toxicol. Sci., May 1, 2005; 85(1): 683 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Antkiewicz, C. G. Burns, S. A. Carney, R. E. Peterson, and W. Heideman Heart Malformation Is an Early Response to TCDD in Embryonic Zebrafish Toxicol. Sci., April 1, 2005; 84(2): 368 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Prasch, W. Heideman, and R. E. Peterson ARNT2 Is Not Required for TCDD Developmental Toxicity in Zebrafish Toxicol. Sci., November 1, 2004; 82(1): 250 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Carney, R. E. Peterson, and W. Heideman 2,3,7,8-Tetrachlorodibenzo-p-dioxin Activation of the Aryl Hydrocarbon Receptor/Aryl Hydrocarbon Receptor Nuclear Translocator Pathway Causes Developmental Toxicity through a CYP1A-Independent Mechanism in Zebrafish Mol. Pharmacol., September 1, 2004; 66(3): 512 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bello, W. Heideman, and R. E. Peterson 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Inhibits Regression of the Common Cardinal Vein in Developing Zebrafish Toxicol. Sci., April 1, 2004; 78(2): 258 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Dong, H. Teraoka, Y. Tsujimoto, J. J. Stegeman, and T. Hiraga Role of Aryl Hydrocarbon Receptor in Mesencephalic Circulation Failure and Apoptosis in Zebrafish Embryos Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Toxicol. Sci., January 1, 2004; 77(1): 109 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hill, C. V. Howard, U. Strahle, and A. Cossins Neurodevelopmental Defects in Zebrafish (Danio rerio) at Environmentally Relevant Dioxin (TCDD) Concentrations Toxicol. Sci., December 1, 2003; 76(2): 392 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Prasch, H. Teraoka, S. A. Carney, W. Dong, T. Hiraga, J. J. Stegeman, W. Heideman, and R. E. Peterson Aryl Hydrocarbon Receptor 2 Mediates 2,3,7,8-Tetrachlorodibenzo-p-dioxin Developmental Toxicity in Zebrafish Toxicol. Sci., November 1, 2003; 76(1): 138 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Zodrow and R. L. Tanguay 2,3,7,8-Tetrachlorodibenzo-p-dioxin Inhibits Zebrafish Caudal Fin Regeneration Toxicol. Sci., November 1, 2003; 76(1): 151 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kiukkonen, M. Viluksela, C. Sahlberg, S. Alaluusua, J. T. Tuomisto, J. Tuomisto, and P.-L. Lukinmaa Response of the Incisor Tooth to 2,3,7,8-Tetrachlorodibenzo-p-dioxin in a Dioxin-Resistant and a Dioxin-Sensitive Rat Strain Toxicol. Sci., October 1, 2002; 69(2): 482 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Tillitt and D. M. Papoulias 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxicity in the Zebrafish Embryo: Local Circulation Failure in the Dorsal Midbrain Is Associated with Increased Apoptosis Toxicol. Sci., September 1, 2002; 69(1): 1 - 2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Dong, H. Teraoka, K. Yamazaki, S. Tsukiyama, S. Imani, T. Imagawa, J. J. Stegeman, R. E. Peterson, and T. Hiraga 2,3,7,8-Tetrachlorodibenzo-p-dioxin Toxicity in the Zebrafish Embryo: Local Circulation Failure in the Dorsal Midbrain Is Associated with Increased Apoptosis Toxicol. Sci., September 1, 2002; 69(1): 191 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Andreasen, J. M. Spitsbergen, R. L. Tanguay, J. J. Stegeman, W. Heideman, and R. E. Peterson Tissue-Specific Expression of AHR2, ARNT2, and CYP1A in Zebrafish Embryos and Larvae: Effects of Developmental Stage and 2,3,7,8-Tetrachlorodibenzo-p-dioxin Exposure Toxicol. Sci., August 1, 2002; 68(2): 403 - 419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Andreasen, M. E. Hahn, W. Heideman, R. E. Peterson, and R. L. Tanguay The Zebrafish (Danio rerio) Aryl Hydrocarbon Receptor Type 1 Is a Novel Vertebrate Receptor Mol. Pharmacol., August 1, 2002; 62(2): 234 - 249. [Abstract] [Full Text] [PDF]
|
|