Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ji, L.
Right arrow Articles by Talbot, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ji, L.
Right arrow Articles by Talbot, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Toxicological Sciences 69, 217-225 (2002)
Copyright © 2002 by the Society of Toxicology

RESPIRATORY TOXICOLOGY

Identification of Pyridine Compounds in Cigarette Smoke Solution That Inhibit Growth of the Chick Chorioallantoic Membrane

Lin Ji, Goar Melkonian, Karen Riveles and Prue Talbot1

Department of Cell Biology and Neuroscience, 135 Spieth Hall, University of California, Riverside, California 92521-0146

Based on prior work, we hypothesized that cigarette smoke contains chemicals that can inhibit growth of the chick chorioallantoic membrane (CAM). In this study, gas chromatography and mass spectrometry were used to identify 12 pyridine derivatives in the inhibitory fractions of smoke eluted from solid phase extraction cartridges. These pyridine derivatives were further studied individually in dose response experiments to determine their effects on CAM growth. A correlation was observed between the functional group substitutions on pyridine and the relative toxicity of each pyridine derivative. In the CAM growth assay, pyridine derivatives with single methyl or single ethyl substitutions had lowest observed adverse effect levels (LOAELs) of 5 x 10-9 and 5 x 10-12 M, respectively. Other pyridine derivatives and pyridine itself had LOAELs in the micromolar range. One of the most inhibitory derivatives, 3-ethylpyridine, was studied further and inhibited cell proliferation, as measured by BrdU incorporation. Since 3-ethylpyridine inhibited growth at picomolar doses and is added to consumer products including cosmetics, food, drinks, and tobacco, it will be important to perform further toxicological testing to determine its effect on human health.

Key Words: pyridine; pyridine derivative; 3-ethylpyridine; growth; chick chorioallantoic membrane; cigarette smoke; passive smoking; active smoking.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
R. Yu, M. Wu, S. Lin, and P. Talbot
Cigarette Smoke Toxicants Alter Growth and Survival of Cultured Mammalian Cells
Toxicol. Sci., September 1, 2006; 93(1): 82 - 95.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
O. Genbacev-Krtolica
Highlight for Phenols, Quinolines, Indoles, Benzene and 2-Cyclopenten-1-ones Are Oviduct Toxicants in Cigarette Smoke, by Prue Talbot, Karen Riveles, and Ryan Rosa: List of Tobacco-Smoke Constituents That Are Harmful for Reproduction Grows--Passive Smokers May Be at Risk
Toxicol. Sci., July 1, 2005; 86(1): 4 - 5.
[Full Text] [PDF]

Home page
 Toxicol SciHome page
G. Melkonian, H. Eckelhoefer, M. Wu, Y. Wang, C. Tong, K. Riveles, and P. Talbot
Growth and Angiogenesis Are Inhibited in Vivo in Developing Tissues by Pyrazine and Its Derivatives
Toxicol. Sci., October 1, 2003; 75(2): 393 - 401.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. M. Rogers and B. D. Abbott
Screening for Developmental Toxicity of Tobacco Smoke Constituents
Toxicol. Sci., October 1, 2003; 75(2): 227 - 228.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.