Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by PRELUSKY, D.B.
Right arrow Articles by MILLER, J.D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by PRELUSKY, D.B.
Right arrow Articles by MILLER, J.D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1986 Oxford University Press

research-article

Tissue Distribution and Excretion of Radioactivity following Administration of 14C-Labeled Deoxynivalenol to White Leghorn Hens

D.B. PRELUSKY*,*, R.M.G. HAMILTON*,*, H.L. TRENHOLM*,* and J.D. MILLER{dagger}

*Animal Research Centre Ottawa, Ontario, Canada KlA 0C6 {dagger}Chemistry Biology Research Institute, Agriculture Canada, Central Experimental Farm Ottawa, Ontario, Canada KlA 0C6

Tissue Distribution and Excretion of Radioactivity following Administration of 14C-Labeled Deoxynivalenol to White Leghorn Hens. PRELUSKY, D.B., HAMILTON, R.M.G., TRENHOLM, H.L., AND MILLER, J.D. (1986). Fundam. Appl. Toxicol. 7, 635-645. The disposition of [14C]deoxynivalenol ([14C]DON) administered to hens as either a single oral dose or consumed in spiked feed over a 6-day period was determined by tracing the specific radioactivity of tissues and excreta. Following a single intubated dose (2.2 mg [14C]DON; 2.4 µCi/bird), the toxin was found to be poorly absorbed; peak plasma levels (2-2.5 hr post-treatment) accounted for less than 1% of the administered dose. Maximum tissue residues were measured at 3 hr in all tissues (liver, kidney, brain, heart, spleen, proventriculus, gizzard, small intestine) except for fat, muscle, and oviduct which occurred at 6 hr postdosing. Among the organs, the highest activities were measured in kidney, liver, and spleen; however, these levels were equal to less than 500 ng DON equivalents/g tissue, and declined quickly. Clearance of radioactivity from tissue had an average half-life of 16.83±8.2 hr (range 7.7-33.3 hr, depending on the tissue). Elimination of the labeled toxin in excreta occurred rapidly; recovery of radioactivity accounted for 78.6,92.1, and 98.5% of the dose by 24,48, and 72 hr, respectively. In continuously dosed birds fed 2.2 mg unlabeled DON for 6 days followed by 2.2 mg (1.5 µCi) [14C]DON for 6 days, accumulation of radioactivity in tissues did not occur. Maximum residual levels, which occurred in the kidneys, were only 60 ng DON equivalents/g. Estimated level of residues contained in the edible tissues amounted to only 13-16 µg DON/1.5 kg hen.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.