Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Chen, S.-C.
Right arrow Articles by Liu, T.-Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, S.-C.
Right arrow Articles by Liu, T.-Z.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Toxicological Sciences 72, 283-288 (2003)
Copyright © 2003 by the Society of Toxicology

MOLECULAR AND GENETIC TOXICOLOGY

Assessment of Genotoxicity of Benzidine and Its Structural Analogues to Human Lymphocytes Using Comet Assay

Ssu-Ching Chen*,1, Chih-Ming Kao{dagger}, Mei-Han Huang{ddagger}, Ming-Kuei Shih§, Ya-Lei Chen{ddagger}, Shiao-Ping Huang{ddagger} and Tsan-Zon Liu

* Department of Medicine, China Medical College, 91 Hsueh-Shih Rd., Taichung 404, Taiwan; {dagger} Institute of Environmental Engineering, National Sun Yat-Sen University, Kaohsiung, Taiwan; {ddagger} Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan; § Department of Food and Beverage Management, National Kaohsiung Hospitality College, Kaohsiung, Taiwan; and Graduate Institute of Medical Biotechnology, Chang Gung University, Taoyan, Taiwan

Benzidine (BZ) and its six structural analogues (2-aminobiphenyl [2-ABP], 4-aminobiphenyl [4-ABP], 3,3'-diaminobenzidine [DABZ], 3,3'-dichlorobenzidine [DCBZ] 3,3'-dimethoxybenzidine [DEBZ], and 3,3'-dimthylbenzidine [DMBZ]) were examined for DNA damage in human lymphocytes using the alkaline comet assay. All the tested compounds showed a distinct disparity in their respective DNA-damaging capacities with an order of DABZ > BZ > DCBZ > 2-ABP > DEBZ > 4-ABP > DMBZ when lymphocytes were exposed to these chemicals for 2 h. Results show that the DNA-damaging effects of these compounds had no bearing on some physicochemical parameters including oxidation potentials, the energy differences between the lowest unoccupied molecular orbital and the highest occupied molecular orbital, ionization potentials, dipole moment, and relative partition coefficient. On the other hand, the free radical scavengers, including catalase, SOD, BHT, EDTA, and histidine exerted varying degrees of inhibitory effects on the DNA damage caused by benzidine. This suggests that genotoxicity in lymphocytes caused by benzidine proceeded via a reactive oxygen species (ROS)-mediated mechanism.

Key Words: benzidine; comet assay; ROS; scavengers.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MutagenesisHome page
C.-Y. Chai, Y.-C. Huang, W.-C. Hung, W.-Y. Kang, and W.-T. Chen
Arsenic salt-induced DNA damage and expression of mutant p53 and COX-2 proteins in SV-40 immortalized human uroepithelial cells
Mutagenesis, November 1, 2007; 22(6): 403 - 408.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.