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© 1987 Oxford University Press

research-article

The Relationship between Benzo(a)pyrene diol-Epoxide-DNA Adducts and Mutagenicity in the CHO/HPGRT Assay1

LESLIE RECIO*,{dagger},2, LEE R. SHUGART{ddagger} and ABRAHAM W. HSIE*,{dagger},3

*University of Kentucky, Graduate Center for Toxicology Lexington, Kentucky 40536 {ddagger}Environmental Sciences Division, Oak Ridge National Laboratory Oak Ridge, Tennessee 37831 {dagger}Health and Safety Research Division, Oak Ridge National Laboratory Oak Ridge, Tennessee 37831 3Department of Preventive Medicine and Community Health, The University of Texas Medical Branch Galveston, Texas 77550

The Relationship Between Benzo(a)pyrene diol-Epoxide–DNA Adducts and Mutagenicity in the CHO/HGPRT Assay. Recio, L., SHUGART, L. R., and HSIE, A. W. (1987). Fundam. Appl. Toxicol. 8, 243–252. We have studied the relationship between DNA adducts in Chinese hamster ovary (CHO) cells and mutagenicity as determined in the CHO/hypoxanthine–guanine phosphoribosyltransferase assay. The cells were treated with benzo(a)pyrene 7,8-diol (BP-diol) in the presence of a bioactivation system, S9 mix. DNA binding by bioactivation of BP-diol with S9 mix occurred with both stereoisomers of benzo(a)pyrene diol-epoxide (BPDE) in approximately equal amounts. The number of BPDE-DNA adducts (21–260 adducts/106 nucleotide base pairs) increased with increasing treatment concentrations of BP-diol (1.4–x7.0 µm). A linear relationship was observed between the number of BPDE-DNA adducts and mutagenicity (89–605 mutants/106 cloneable cells) over the concentration range of BP-diol assayed.


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