ToxSci Advance Access originally published online on October 13, 2004
Toxicological Sciences 2005 83(1):155-165; doi:10.1093/toxsci/kfi007
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Toxicological Sciences vol. 83 no. 1 © Society of Toxicology 2005; all rights reserved.
Inhaled Diesel Engine Emissions Reduce Bacterial Clearance and Exacerbate Lung Disease to Pseudomonas aeruginosa Infection In Vivo
* Asthma and Pulmonary Immunology Program, Experimental Toxicology Program, Lovelace Respiratory Research Institute, Albuquerque, New Mexico and SKS Consulting Services, Siler City, North Carolina
Received April 20, 2004; accepted September 26, 2004
Despite experimental evidence supporting an adverse role for air pollution in models of human disease, little has been done in the way of assessing the health effects of inhalation of whole mixtures from defined sources at exposure levels relevant to ambient environmental exposures. The current study assessed the impact of inhaled diesel engine emissions (DEE) in modulating clearance of Pseudomonas aeruginosa (P.a.) and the adverse effects of infection to the pulmonary epithelium. At DEE concentrations representing from high ambient to high occupational exposures, mice were exposed to DEE continuously for one week or six months (6 h/day), and subsequently infected with P.a. by intratracheal instillation. At 18 h following P.a. infection, prior exposure to DEE impaired bacterial clearance and exacerbated lung histopathology during infection. To assess the airway epithelial cell changes indicative of lung pathogenesis, markers of specific lung epithelial cell populations were analyzed by immunohistochemistry. Both ciliated and non-ciliated airway epithelial cell numbers were decreased during P.a. infection by DEE exposure in a concentration-dependent manner. Furthermore, the lung transcription regulator, thyroid transcription factor 1 (TTF-1), was also decreased during P.a. infection by prior exposure to DEE concordant with changes in airway populations. These findings are consistent with the notion that environmental levels of DEE can decrease the clearance of P.a. and increase lung pathogenesis during pulmonary bacterial infection.
Key Words: respiratory infection; pulmonary toxicology; air pollution.
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