Skip Navigation


ToxSci Advance Access originally published online on November 10, 2004
Toxicological Sciences 2005 84(2):278-286; doi:10.1093/toxsci/kfi032
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
84/2/278    most recent
kfi032v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yancy, S. L.
Right arrow Articles by Welsh, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yancy, S. L.
Right arrow Articles by Welsh, M. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Toxicological Sciences vol. 84 no. 2 © Society of Toxicology 2005; all rights reserved.

HIGHLIGHTED ARTICLE

Sodium Arsenite Exposure Alters Cell Migration, Focal Adhesion Localization and Decreases Tyrosine Phosphorylation of Focal Adhesion Kinase in H9C2 Myoblasts

Shannon L. Yancy*, Eric A. Shelden{dagger}, Robert R. Gilmont{ddagger} and Michael J. Welsh§,1

* Toxicology Department, School of Public Health, University of Michigan, Ann Arbor, Michigan; {dagger} Department of Molecular Biosciences, Washington State University, Pullman, Washington; {ddagger} Department of Surgery, Medical School, University of Michigan, Ann Arbor, Michigan; and § Department of Cell and Developmental Biology, Medical School, University of Michigan, Ann Arbor, Michigan

Received September 16, 2004; accepted November 6, 2004

Exposure to the environmental toxicant arsenic is reported to produce a variety of effects including disruption of signal transduction pathways, cell proliferation, and apoptosis. This suggests that arsenite may not have specific targets but rather extremely broad effects. The present study was designed to test the hypothesis that arsenite alters signaling involved in focal adhesion structure and function in cultured myoblasts. H9C2 cells were exposed to 1, 2.5, 5, or 10 µM sodium arsenite for 48 h. MTT metabolism and staining by neutral red, trypan blue, and propidium iodide showed that sodium arsenite treatments of 5 µM or less were not overtly cytotoxic. At these doses, sodium arsenite did not affect the amount of polymerized actin in cells, rate of protein synthesis, or amounts of vinculin, talin, paxillin, and focal adhesion kinase (FAK) in cells. However, sodium arsenite-treated cells contained fewer focal adhesions with an altered distribution pattern. Sodium arsenite exposure caused a dose-dependent reduction in cell migration and cell attachment rates. The average area of substrate covered by a cell was also reduced, although the average volume of cells was not significantly affected. Sodium arsenite exposure resulted in reduced tyrosine phosphorylation of FAK, its substrate paxillin and the FAK auto- phosphorylation site, Tyr397. Our results indicate that sodium arsenite can alter focal adhesion structure and function, thus affecting cell attachment and migration and possibly other aspects of focal adhesion function such as integrin signaling. These diverse consequences may be mediated by a relatively specific inhibition of FAK tyrosine phosphorylation, modifying scaffolding proteins.

Key Words: sodium arsenite; focal adhesions; FAK; paxillin; phosphotyrosine; cell migration.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. E. Olsen, A. E. Liguori, Y. Zong, R. C. Lantz, J. L. Burgess, and S. Boitano
Arsenic upregulates MMP-9 and inhibits wound repair in human airway epithelial cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L293 - L302.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.