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ToxSci Advance Access originally published online on September 21, 2005
Toxicological Sciences 2005 88(2):545-550; doi:10.1093/toxsci/kfi333
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© The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Protective Effect of Quercetin on Aroclor 1254–Induced Oxidative Damage in Cultured Chicken Spermatogonial Cells

Yuling Mi Caiqiao Zhang1

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Received August 22, 2005; accepted September 15, 2005

Quercetin, a dietary-derived falvonol-type flavonoid, is ubiquitous in fruits and vegetables and plays important roles in human health by virtue of its antioxidant function. The present study was performed to investigate effects of quercetin on oxidative damage that was induced by an environmental endocrine disrupter, Aroclor 1254 (A1254), in cultured spermatogonial cells of embryonic chickens. Spermatogonial cells were dispersed from 18-day-old embryo and exposed to A1254 alone or in combination with quercetin. The oxidative damage was estimated by measuring contents of thiobarbituric acid–reactive substances (TBARS, an indicator of lipid peroxidation), activity of superoxide dismutase (SOD, a scavenger of superoxide), and activity of glutathione (GSH, an intracellular antioxidant). Results showed that quercetin had no deleterious effect on spermatogonial cells at 0.01~1 µg/ml. Exposure to A1254 (10 µg/ml) induced an increase of spermatogonial cell number, and membrane integrity was damaged by elevation of lactate dehydrogenase (LDH) leakage. Exposure to A1254 also induced an elevation in TBARS but a decrease in SOD activity and GSH content. However, compared with A1254 treatment alone, simultaneous supplementation with quercetin decreased LDH leakage to maintain the cell integrity, decreased the levels of TBARS to quench the free radicals, increased SOD activity and GSH content to restore the endogenous antioxidant defense system. Thus, quercetin displayed protective effects on spermatogonial cells from A1254-induced oxidative damage through increasing intracellular antioxidant levels and decreasing lipid peroxidation. Consequently, the antioxidant, such as quercetin, from food or feed consumed by human and animals may attenuate the negative effects of environmental endocrine disrupters.

Key Words: quercetin; polychlorinated biphenyls; oxidative damage; chicken; spermatogonial cells.


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