Skip Navigation


ToxSci Advance Access originally published online on April 6, 2006
Toxicological Sciences 2006 92(1):157-173; doi:10.1093/toxsci/kfj187
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
92/1/157    most recent
kfj187v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (68)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Hamers, T.
Right arrow Articles by Brouwer, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamers, T.
Right arrow Articles by Brouwer, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

In Vitro Profiling of the Endocrine-Disrupting Potency of Brominated Flame Retardants

Timo Hamers*,1, Jorke H. Kamstra*, Edwin Sonneveld{dagger}, Albertinka J. Murk{ddagger}, Monique H. A. Kester§, Patrik L. Andersson, Juliette Legler* and Abraham Brouwer*,{dagger}

* Institute for Environmental Studies, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands; {dagger} BioDetection Systems BV, 1098 SM Amsterdam, The Netherlands; {ddagger} Toxicology Section, Wageningen University, 6703 HE Wageningen, The Netherlands; § Erasmus University Medical Center, 3015 GJ Rotterdam, The Netherlands; and Environmental Chemistry, Umeå University, SE-901 87 Umeå, Sweden

Received February 1, 2006; accepted April 1, 2006

Over the last few years, increasing evidence has become available that some brominated flame retardants (BFRs) may have endocrine-disrupting (ED) potencies. The goal of the current study was to perform a systematic in vitro screening of the ED potencies of BFRs (1) to elucidate possible modes of action of BFRs in man and wildlife and (2) to classify BFRs with similar profiles of ED potencies. A test set of 27 individual BFRs were selected, consisting of 19 polybrominated diphenyl ether congeners, tetrabromobisphenol-A, hexabromocyclododecane, 2,4,6-tribromophenol, ortho-hydroxylated brominated diphenyl ether 47, and tetrabromobisphenol-A–bis(2,3)dibromopropyl ether. All BFRs were tested for their potency to interact with the arylhydrocarbon receptor, androgen receptor (AR), progesterone receptor (PR), and estrogen receptor. In addition, all BFRs were tested for their potency to inhibit estradiol (sulfation by estradiol sulfotransferase (E2SULT), to interfere with thyroid hormone 3,3',5-triiodothyronine (T3)–mediated cell proliferation, and to compete with T3-precursor thyroxine for binding to the plasma transport protein transthyretin (TTR). The results of the in vitro screening indicated that BFRs have ED potencies, some of which had not or only marginally been described before (AR antagonism, PR antagonism, E2SULT inhibition, and potentiation of T3-mediated effects). For some BFRs, the potency to induce AR antagonism, E2SULT inhibition, and TTR competition was higher than for natural ligands or clinical drugs used as positive controls. Based on their similarity in ED profiles, BFRs were classified into five different clusters. These findings support further investigation of the potential ED effects of these environmentally relevant BFRs in man and wildlife.

Key Words: toxicity profiling; brominated flame retardants; endocrine disruption; hierarchical cluster analysis; principal component analysis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Phil Trans R Soc BHome page
C. E. Talsness, A. J. M. Andrade, S. N. Kuriyama, J. A. Taylor, and F. S. vom Saal
Components of plastic: experimental studies in animals and relevance for human health
Phil Trans R Soc B, July 27, 2009; 364(1526): 2079 - 2096.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. M. Weiss, P. L. Andersson, M. H. Lamoree, P. E. G. Leonards, S. P. J. van Leeuwen, and T. Hamers
Competitive Binding of Poly- and Perfluorinated Compounds to the Thyroid Hormone Transport Protein Transthyretin
Toxicol. Sci., June 1, 2009; 109(2): 206 - 216.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
F. Ucan-Marin, A. Arukwe, A. Mortensen, G. W. Gabrielsen, G. A. Fox, and R. J. Letcher
Recombinant Transthyretin Purification and Competitive Binding with Organohalogen Compounds in Two Gull Species (Larus argentatus and Larus hyperboreus)
Toxicol. Sci., February 1, 2009; 107(2): 440 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. M. L. Dingemans, H. J. Heusinkveld, A. de Groot, A. Bergman, M. van den Berg, and R. H. S. Westerink
Hexabromocyclododecane Inhibits Depolarization-Induced Increase in Intracellular Calcium Levels and Neurotransmitter Release in PC12 Cells
Toxicol. Sci., February 1, 2009; 107(2): 490 - 497.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
D. Crump, S. Chiu, C. Egloff, and S. W. Kennedy
Effects of Hexabromocyclododecane and Polybrominated Diphenyl Ethers on mRNA Expression in Chicken (Gallus domesticus) Hepatocytes
Toxicol. Sci., December 1, 2008; 106(2): 479 - 487.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
L. T. M. van der Ven, A. Verhoef, T. van de Kuil, W. Slob, P. E. G. Leonards, T. J. Visser, T. Hamers, M. Herlin, H. Hakansson, H. Olausson, et al.
A 28-Day Oral Dose Toxicity Study Enhanced to Detect Endocrine Effects of Hexabromocyclododecane in Wistar Rats
Toxicol. Sci., December 1, 2006; 94(2): 281 - 292.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.