Skip Navigation



ToxSci Advance Access published online on October 4, 2009
Toxicological Sciences, doi:10.1093/toxsci/kfp239
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Braun, A.
Right arrow Articles by Mempel, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braun, A.
Right arrow Articles by Mempel, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Differential impact of diesel particle composition on pro-allergic dendritic cell function

Andrea Braun*, Mayte Bewersdorff*, Jutta Lintelmann{dagger}, Georg Matuschek{dagger}, Thilo Jakob{ddagger}, Martin Göttlicher§, Wolfgang Schober*, Jeroen T. M. Buters*, Heidrun Behrendt* and Martin Mempel*,¶

* ZAUM - Center for Allergy and Environment, Technische Universität München (TUM), Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, 80802 Munich, Germany {dagger} Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Ecological Chemistry, 85764 Neuherberg, Germany {ddagger} Allergy Research Group, Department of Dermatology, University Medical Center Freiburg, 79104 Freiburg, Germany § Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Toxicology, 85764 Neuherberg, Germany Department of Dermatology and Allergy Biederstein, Technische Universität München, 80802 Munich, Germany

Corresponding author: Martin Mempel, Helmholtz Zentrum München, Division of Environmental Dermatology and Allergy, Building 34, Room 230, Ingolstädter Landstraße 1, 85764 Neuherberg, Phone: +49/89/3187/2556, Fax: +49/89/3187/2540, e-mail address: martin.mempel{at}lrz.tu-muenchen.de

Received June 18, 2009; revision received September 2, 2009; accepted September 15, 2009


  Abstract

Diesel exhaust particles (DEP) were described as potent adjuvant in the induction and maintenance of allergic diseases suggesting that they might play a role in the increase of allergic diseases in the industrialized countries. However, the cellular basis by which these particles enhance allergic immune responses is still a matter of debate.

Thus, we exposed immature murine bone marrow-derived dendritic cells (BMDC) to different particles or particle-associated organic compounds in the absence or presence of the maturation stimuli lipopolysachharide and analyzed the cellular maturation, viability, and cytokine production. Furthermore, we monitored the functionality of particle-exposed BMDC to suppress B cell isotype switching to IgE.

Only highly polluted DEP (standard reference material SRM1650a) but not particle-associated organic compounds or less polluted DEP from modern diesel engines were able to modulate the dendritic cell phenotype. SRM1650a particles significantly suppressed LPS-induced IL-12p70 production in murine BMDC, whereas cell surface marker expression was not altered. Furthermore, SRM1650a-exposed immature BMDC lost the ability to suppress IgE isotype switch in B cells.

This study revealed that highly polluted DEP not only interfere with dendritic cell maturation but additionally with dendritic cell function, thus suggesting a role in Th2 immune deviation.

Key Words: Dendritic cells; B cells; IgE; particles; organic compounds.

e-mail addresses: andrea.braun{at}helmholtz-muenchen.de, mayte.b{at}gmx.de, matuschek{at}helmholtz-muenchen.de, thilo.jakob{at}uniklinik-freiburg.de, martin.goettlicher{at}helmholtz-muenchen.de, wolfgang.schober{at}lrz.tu-muenchen.de, buters{at}lrz.tum.de, heidrun.behrendt{at}lrz.tum.de, martin.mempel{at}lrz.tu-muenchen.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.