Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by MONTEIRO-RIVIERE, N. A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by MONTEIRO-RIVIERE, N. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1990 Oxford University Press

research-article

Altered Epidermal Morphology Secondary to Lidocaine Iontophoresis: In Vivo and in Vitro Studies in Porcine Skin

NANCY A. MONTEIRO-RIVIERE

Cutaneous Pharmacology and Toxicology Center, North Carolina State University 4700 Hillsborough Street, Raleigh, North Carolina 27606

Received October 30, 1989; accepted February 13, 1990

Altered Epidermal Morphology Secondary to Lidocaine Iontophoresis: In Vivo and in Vitro Studies in Porcine Skin. MONTEIRO-RIVIERE, N. A. (1990). Fundam Appl. Toxicol 15, 174–185. Iontophoresis is the process of delivering ionic drugs across the skin using electric current. Iontophoresis of lidocaine hydrochloride in 30 pigs in vivo and in 112 in vitro isolated perfused porcine skin flap (IPPSF) preparations produced a drug-specific alteration in the epidermis ≥ 10 mm after dosing. By light microscopy, this change was characterized by the appearance of flattened dark basophilic staining nuclei oriented parallel to the stratum corneum in the stratum granulosum and spinosum layers. In severe cases, this alteration extended into the deeper usually vacuolated stratum basale. The stratum corneum appeared normal. This unique morphological alteration showed an abrupt change from the stratum basale to stratum granulosum. An immune-mediated etiology can be ruled out since this alteration is observed both in vivo and in vitro. The severity of this change, graded on a scale of 0–3 (no change to severe), was best corre lated to total transcutaneous lidocaine flux as estimated in IPPSF studies and to flux as estimated by current (mA-hr) in vivo. Electron microscopic changes following iontophoresis showed specific alterations in the tonofilaments of the epidermal cells. The tonofilaments appeared unrecognizable and resembled an amorphous matnx. In pigs followed through 10 days to study the resolution of this alteration, the epidermis reverted to normal within 6 days with no additional manifestations. In conclusion, lidocaine iontophoresis can induce in swine a unique dose-dependent non-immune-mediated epidermal alteration which is expected to have minimal toxicological significance.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.