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© 1991 Oxford University Press

research-article

Reproductive Toxicity Assessment by Continuous Breeding in Sprague-Dawley Rats: A Comparison of two Study Designs

D. K. GULATI, E. HOPE, J. TEAGUE* and R. E. CHAPIN{dagger}

Environmental Health Research and Testing 2514 Regency Road, Kentucky 40503 *Analytical Sciences, Inc. Suite 106, 100 Capitola Drive, Durham, North Carolina 27713 {dagger}Development and Reproductive Toxicology Group, NIEHS/National Toxicology Program P O. Box 12233, Research Triangle Park, North Carolina 27709

Received October 15, 1990; accepted February 18, 1991

Reproductive Toxtclty Assessment by Continuous Breeding in Sprague-Dawley Rats: A Comparison of Two Study Designs. GULATI, D. K., HOPE, E., TEAGUE, J. AND CHAPIN, R. E. (1991). Fundam. Appl. Toxicol 17, 270–279. The protocol for Reproductive Assessment by Continuous Brecding (RACB) studies was originally designed to use mice as the test species. However, rats are commonly used for reproductive toxicity research and could be used in the basic RACB design. One of the studies reported below evaluated a standard murine RACB design using rats, which rears the fifth litter to test second generation fertility. The second design tested the logistics and feasibility of rearing the second liner for second generation fertility testing. The standard fifth litter design (L5) was modified slightly for rats by increasing the time allowed for gestation and delivery. Compared to rats rearing their second litter (L2), rats in this L5 design had more litters per pair during continuous breeding and maintained this fertility better over time, as evidenced by producing more pups per litter during the crossover mating segment Both L2 and L5 rats gave sufficient pups to conduct the second generation fertility evaluation. In addition, the L5 design was easier to conduct and produced pups from gametes exposed to chemical throughout spermatogenesis making it the preferred design for using rats in Continuous Breeding studies.


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