© 1982 Oxford University Press
research-article |
Metabolism of 
-Olefin Sulfonate (AOS) in Rats
Biological Science Laboratory, Lion Corporation Odawara, Kanagawa 256, Japan ADepartment of Metabolism and Analytical Chemistry, Bio/dynamics Inc. East Millstone, New Jersey 08873, U.S.A. BDepartment of Pharmacology, Kitasato University Minato-ku, Tokyo 108, Japan
Metabolism of 
-Olefin Sulfonate (AOS) in Rats. Inoue, S., O'Grodnick, J.S. and Tomizawa, S. (1982). Fundam. Appl. Toxicol. 2:130-138. The metabolic fate of 14C-AOS (a mixture of 14C-sodium alkenyl(2) sulfonate and 14C-sodium 3-hydroxy alkane sulfonate) has been studied in rats by a single oral and intravenous injection of 100 mg (50 µCi)/kg and 10 mg (5 µCi)/kg, respectively. After oral administration, 14C-AOS was rapidly absorbed from the gastrointestinal tract. The blood level of 14C-activity reached its peak 3 hr after dosing and then declined. Twenty-four hr after the dose, about 0.8%/g of the 14C-AOS given was detected in cecum content, but in other tissues the figures were under 0.02% dose/g. Within 24 hr after the dose, 72% of the dose was excreted in the urine and 22% in the feces, while the excretion in the bile was 4.3% within 12 hr. The administered radioactivity was rapidly eliminated from the whole body within 24 hr. After intravenous injection, half of the administered dose of radioactivity was excreted within 1 hr. In the 0–6 hr interval post-dose, 90% of the dose was excreted in the urine. No intact 14C-AOS was detected in any of the urine samples after oral and intravenous doses. The metabolite was apparently more polar than intact 14C-AOS, and results from data of electrophoresis and equilibrium dialysis indicated that intact 14C-AOS can bind with proteins, while the metabolites cannot. The metabolite was found to contain alcoholic, unsaturated and sulfonic acid functionalities. It is suggested that the metabolite may be a hydroxylated or polyhydroxylated sulfonic acid of shorter chain length than AOS. These results suggest that 14C-AOS is rapidly absorbed, metabolized and excreted, therefore, no accumulation of 14C-AOS occurs.