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© 1994 Oxford University Press

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Absence of Detectable P450 2E1 in Bone Marrow of B6C3F1 Mice: Relevance to Butadiene-Induced Bone Marrow Toxicity1

MARY BETH GENTER*,2 and LESLIE RECIO{dagger}

*Department of Toxicology, North Carolina State University Box 7633, Raleigh, North Carolina 27695 {dagger}Department of Cellular and Molecular Toxicology, Chemical Industry Institute of Toxicology P.O. Box 12137, Research Triangle Park, North Carolina 27709

Received July 12, 1993; accepted October 28, 1993

The observation that the concentration of butadiene monoepoxide (BMO) is greater in bone marrow than in the blood following inhalation of 1,3-butadiene (BD) by B6C3F1 mice prompted the present investigation into whether cytochrome P450 2E1, the isozyme believed to be involved in the epoxidation of BD, is present in the bone marrow of B6C3F1 mice. The bone marrow of male and female B6C3F1 mice was analyzed by Western blot analysis and immunohistochemistry to determine whether cytochrome P450 2E1 is present, and, if present, in which cell types. Both Western blot and immunohistochemical analyses revealed the apparent absence of P450 2E1 in B6C3F1 mouse bone marrow. This observation suggests that BD is converted to BMO outside of bone marrow and is subsequently concentrated in the bone marrow, or that the conversion of BD to BMO occurs by an alternate enzymatic pathway within the bone marrow.


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