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© 1996 Oxford University Press

research-article

Ultrastructural, Protein, and Lipid Changes in Liver Associated with Chlordecone Treatment of Mice1,2

HILLARY M. CARPENTER*,3, OLAF R. HEDSTROM{dagger}, LISBETH K. SIDDENS*, JULIE R. DUIMSTRA{dagger}, ZHENG-WEI CAI*,4, KAY A. FISHER{dagger} and LAWRENCE R. CURTIS*,{ddagger},5

*Oak Creek Laboratory of Biology, Oregon State University Corvallis, Oregon 97331 {dagger}College of Veterinary Medicine, Oregon State University Corvallis, Oregon 97331 {ddagger}East Tennessee State University Johnson City, Tennessee 37614-0682

Received May 23, 1996; accepted August 21, 1996

Pretreatment of mice with chlordecone (CD) reduced hepatic accumulation of a subsequent dose of [14C]CD without significantly changing [14C]CD biotransformation. To determine if CD-induced changes in hepatic [14C]CD accumulation were coincident with altered cell composition, we examined the effects of CD on hepatic protein and lipid content, on fatty acid profiles of liver and kidney, and on the ultrastructure of hepatocytes. SDS-polyacrylamide gel electrophoresis detected an apparent CD dose-related increase in a microsomal protein with a molecular weight of about 23 kDa. Total liver or kidney lipid contents were not altered by CD but relative amounts of several hepatic fatty acids were changed. CD caused marked hepatic mitochondrial swelling, increased amounts of endoplasmic reticulum, apparently increased numbers of peroxisome-like structures, and decreased numbers of lipid droplets in cytoplasm of hepatocytes. Numbers of lipid droplets were not decreased in perisinusoidal fat storage cells. In addition, the numbers of cytoplasmic lipoprotein vesicles were apparently increased in some hepatocytes. Overall these changes indicated an increased hepatocyte secretory activity and suggested that CD changed hepatocellular lipid transport, storage, and metabolism pathways.


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