Toxicological Sciences 54, 374-383 (2000)
Copyright © 2000 by the Society of Toxicology
Hemopoietic Progenitor Cells Are Sensitive Targets of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in C57BL/6J Mice
Department of Environmental Medicine, University of Rochester, Rochester, New York 14642
Treatment of adult C57BL/6J mice with tetrachlorodibenzo-p-dioxin (TCDD) elicits altered bone marrow hemopoietic cellular potentials and markedly reduced T-lymphoid-reconstituting activity. The latter has been hypothesized to play a role in TCDD-induced thymic atrophy. To investigate cellular targets responsible for reduced prothymocyte capacity, bone marrow cells from TCDD-treated C57BL/6J mice were assessed for hemopoietic alterations within the lineage-negative (lin–) compartment by the examination of Sca-1 and c-Kit levels. Lin– hemopoietic cells from C57BL/6J mice, treated with 30 µg/kg of TCDD, were assessed for phenotypic alterations following 24 h through 31 days. The responses of lin– cells to TCDD doses ranging from 0.3 to 30 µg/kg were also assessed at 2 days following TCDD treatment. The data reveal increases in the number of bone marrow lin– Sca-1+ c-Kit+ cells, relative to control, over 24 h through 31 days following treatment, as well as dose-dependent increases in this population when examined at 2 days. Increases in lin– Sca-1+ c-Kit– cells occurred on a more transient basis and were also dependent upon TCDD dose. These data suggest that proliferation and/or differentiation processes of hemopoietic stem cells are affected by TCDD and that these effects contribute to a reduced capacity of bone marrow to generate pro-T lymphocytes.
Key Words: tetrachlorodibenzo-p-dioxin; TCDD; hemopoiesis; Sca-1; c-Kit; hemopoietic stem cells; flow cytometry.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. W. Garrett and T. A. Gasiewicz The Aryl Hydrocarbon Receptor Agonist 2,3,7,8-Tetrachlorodibenzo-p-dioxin Alters the Circadian Rhythms, Quiescence, and Expression of Clock Genes in Murine Hematopoietic Stem and Progenitor Cells Mol. Pharmacol., June 1, 2006; 69(6): 2076 - 2083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nohara, X. Pan, S.-i. Tsukumo, A. Hida, T. Ito, H. Nagai, K. Inouye, H. Motohashi, M. Yamamoto, Y. Fujii-Kuriyama, et al. Constitutively Active Aryl Hydrocarbon Receptor Expressed Specifically in T-Lineage Cells Causes Thymus Involution and Suppresses the Immunization-Induced Increase in Splenocytes J. Immunol., March 1, 2005; 174(5): 2770 - 2777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Laiosa, A. Wyman, F. G. Murante, N. C. Fiore, J. E. Staples, T. A. Gasiewicz, and A. E. Silverstone Cell Proliferation Arrest within Intrathymic Lymphocyte Progenitor Cells Causes Thymic Atrophy Mediated by the Aryl Hydrocarbon Receptor J. Immunol., November 1, 2003; 171(9): 4582 - 4591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sakai, T. Kajiume, H. Inoue, R. Kanno, M. Miyazaki, Y. Ninomiya, and M. Kanno TCDD Treatment Eliminates the Long-Term Reconstitution Activity of Hematopoietic Stem Cells Toxicol. Sci., March 1, 2003; 72(1): 84 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Laiosa, Z.-W. Lai, T. S. Thurmond, N. C. Fiore, C. DeRossi, B. C. Holdener, T. A. Gasiewicz, and A. E. Silverstone 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Alterations in Lymphocyte Development and Thymic Atrophy in Hemopoietic Chimeras Generated from Mice Deficient in ARNT2 Toxicol. Sci., September 1, 2002; 69(1): 117 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Thurmond and T. A. Gasiewicz A Single Dose of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Produces a Time- and Dose-Dependent Alteration in the Murine Bone Marrow B-Lymphocyte Maturation Profile Toxicol. Sci., November 1, 2000; 58(1): 88 - 95. [Abstract] [Full Text] [PDF]
|
|