Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by GOLBERG, L.
Right arrow Search for Related Content
PubMed
Right arrow Articles by GOLBERG, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1986 Oxford University Press

other

Charting a Course for Cell Culture Alternatives to Animal Testing1

LEON GOLBERG

Division of Occupazio Medicine, Duke University Medical Center Durham. North Carolina 27710

Charting a Course for Cell Culture Alternatives to Animal Testing. GOLBERG, L (1986). Fundam. Appl. Toxicol. 6, 607–617. Cell culture systems (CCS) are finding increasing use in toxicology. They supplement and amplify existing tests in animals, and play a special role in the iterative process involved in studies of mechanisms of toxic action. The need is stressed for adequate standardization and validation of CCS, and meticulous conduct of tests using such systems. A review of some examples of current work with CCS highlights "designer" CCS, primary systems developed for research on morphological, biochemical, and other aspects of toxic action at particular target sites. An account of the use of CCS in studying the stepwise process of neoplastic trans formation focuses on C3H/10T1/2C18 cells in regard to initiation and promotion. CCS of human origin reveal the anticipated intenndividual differences in metabolic characteristics and susceptibility to toxic action. Human CCS, especially primary epidermal keratinocytes, are making important contributions to understanding the actions of dioxins. Reasons for the continuing need for whole animals in toxicology are analyzed, to reveal some strategies by which CCS may prove increasingly successful as replacements.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.