Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by RIVIERE, J. E.
Right arrow Articles by CARVER, M. P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by RIVIERE, J. E.
Right arrow Articles by CARVER, M. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1986 Oxford University Press

research-article

The Isolated Perfused Porcine Skin Flap (IPPSF)

I. A Novel in Vitro Model for Percutaneous Absorption and Cutaneous Toxicology Studies

J. EDMOND RIVIERE*,1, KARL F. BOWMAN{dagger}, NANCY A. MONTEIRO-RIVIERE{ddagger}, LYNN P. Dix* and MICHAEL P. CARVER*

*Laboratory of Toxicokinetics, Department of Anatomy, Physiological Sciences, and Radiology, and Interdepartmental Toxicology Program North Carolina State University, Raleigh, North Carolina 27606 {dagger}Laboratory of Surgical Research, Department of Food Animal and Equine Medicine North Carolina State University, Raleigh, North Carolina 27606 {ddagger}Laboratory of Comparative Dermatology, Department of Anatomy, Physiological Sciences, and Radiology, and Interdepartmental Toxicology Program, School of Veterinary Medicine North Carolina State University, Raleigh, North Carolina 27606

The Isolated Perfused Porcine Skin Flap (IPPSF). I.A Novel in Vitro Model forPercutaneous Absorption and Cutaneous Toxicology Studies. RIVIERE, J.E., BOWMAN, K.F., MONTEIRO-RIVIERE, N.A., Dix, L.P., AND CARVER, M.P. (1986). Fundam. Appl. Toxicol 7, 444-453. This article describes the development of a novel in vitro alternative animal model for dermatology and cutaneous toxicology. A single-pedicle, axial-pattern, island-tubed skin flap was created in crossbred Yorkshire weanling pigs in one surgical procedure, then transferred 2 or 6 days later to a computer-controlled temperature-regulated perfusion chamber for 10- to 12-hr studies. Perfusate consisted of Krebs-Ringer bicarbonate buffer (pH 7.4) containing albumin and glucose. Viability was assessed by glucose utilization, lactate production, an absence of significant concentrations of the intracellular enzyme lactate dehydrogenase in the perfusate, and light and electron microscopy. A mean lactate to glucose ratio of 1.6 for flaps harvested 2 days after surgery and 1.8 for flaps taken 6 days after surgery suggested primarily anaerobic glycolysis. This preparation would be a humane alternative animal model for studies in cutaneous toxicology, physiology, oncology, and percutaneous drug absorption and metabolism.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.