ToxSci Advance Access originally published online on September 21, 2005
Toxicological Sciences 2005 88(2):447-455; doi:10.1093/toxsci/kfi325
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Inhibition and Induction of Aromatase (CYP19) Activity by Brominated Flame Retardants in H295R Human Adrenocortical Carcinoma Cells
* Institute for Risk Assessment Sciences, IRAS Utrecht University, Utrecht, The Netherlands; Institut National de la Recherche Scientifique, Institut Armand-Frappier (INRS-IAF), Université du Québec, Montréal, Québec, H9R 1G6, Canada; National Wildlife Research Centre, Canadian Wildlife Service, Environment Canada, Ottawa, Ontario K1A 0H3 Canada; Department of Environmental Chemistry and Analytical Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden
Received August 4, 2005; accepted September 12, 2005
Brominated flame retardants (BFRs) are persistent and ubiquitous chemicals in the environment, and they are found at increasing levels in tissues of wildlife and humans. Previous in vitro studies with the BFR class of polybrominated diphenyl ethers (BDEs) have shown endocrine-disrupting properties. Our study assessed the potential effects of nineteen BDEs, five hydroxylated BDEs (OH-BDEs), one methoxylated BDE (CH3O-BDE), tetrabromobisphenol-A (TBBPA), its dibromopropane ether derivative (TBBPA-DBPE), and the brominated phenols/anisols 2,4,6-tribromophenol (TBP), 4-bromophenol (4BP) and 2,4,6-tribromoanisole (TBA) on the catalytic activity of the steroidogenic enzyme aromatase (CYP19) in H295R human adrenocortical carcinoma cells. Effects were studied in the concentration range from 0.5 to 7.5 µM; exposures were for 24 h. Both 6-OH-BDE47 and 6-OH-BDE99 showed an inhibitory effect on aromatase activity at concentrations >2.5 µM and >5 µM, respectively. However, 6-OH-BDE47 also caused a statistically significant increase in cytotoxicity (based on mitochondrial MTT reduction and lactate dehydrogenase-leakage [LDH]) at concentrations >2.5 µM that could explain in part the apparent inhibitory effect on aromatase activity. Compared to 6-OH-BDE47, the methoxy analog (6-CH3O-BDE47) did not elicit a cytotoxic effect, whereas significant inhibition of aromatase remained. TBP caused a concentration-dependent induction of aromatase activity between 0.5 and 7.5 µM (with a maximum of 3.8-fold induction at 7.5 µM). This induction was not observed when a OH– group replaced the CH3O– group or when bromine atoms adjacent to this OH– group were absent. These in vitro results provide a basis for studies of more detailed structure–activity relationships between these brominated compounds and the modulation of aromatase activity.
Key Words: brominated flame retardants (BFRs); BDEs; OH-PBDEs; CH3O-PBDEs; aromatase (CYP19); human adrenocortical (H295R) cell line.
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