ToxSci Advance Access originally published online on May 21, 2007
Toxicological Sciences 2007 98(2):427-435; doi:10.1093/toxsci/kfm126
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Globin S-Propyl Cysteine and Urinary N-Acetyl-S-Propylcysteine as Internal Biomarkers of 1-Bromopropane Exposure
* Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee 37232-2561
Shanghai Institute of Planned Parenthood Research, WHO Collaborating Center for Research Reproduction, Shanghai 200032, China
Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan
1 To whom correspondence should be addressed at Department of Pathology, Vanderbilt University Medical Center, 1161 21st Ave S., Nashville, Tennessee 37232-2561. Fax: (615) 343-9825. E-mail: holly.valentine{at}vanderbilt.edu.
Received May 4, 2007; accepted May 8, 2007
 |
Abstract |
|---|
1-Bromopropane (1-BP), an alternative to ozone-depleting solvents, is a neuro and reproductive toxicant in animals and humans. In this study, the dose responses for urinary AcPrCys and S-propylcysteine (PrCys) adducts on globin and neurofilaments were determined as a function of 1-BP exposure level and duration in the rat; and globin PrCys adducts and urinary AcPrCys were quantified in samples obtained from workers in a 1-BP production facility. Rats were exposed to 1-BP by inhalation for 2 weeks at 0, 50, 200, or 800 ppm and to 1-BP at 0 or 50 ppm for 4 weeks. After the 4-week exposures ended, half of the animals were euthanized immediately and half euthanized 8 days later. Urinary AcPrCys was measured using liquid chromatography–tandem mass spectrometry (LC/MS/MS) and gas chromatograph–mass spectrometry (GC/MS); and PrCys adducts were determined on globin and neurofilaments using LC/MS/MS. In rats, PrCys adduct and urinary AcPrCys levels demonstrated a linear dose response relative to exposure level. PrCys globin adducts demonstrated a linear cumulative dose response over the 4-week exposure period. Elimination of AcPrCys appeared biphasic with detectable levels still present in urine up to 8 days postexposure. A significant increase in globin PrCys adducts was observed in the 1-BP workers relative to control workers; and urinary AcPrCys increased with increasing 1-BP ambient exposure levels. The results of these studies demonstrate the ability of 1-BP to covalently modify proteins in vivo and support the potential of urinary AcPrCys and globin PrCys adducts to serve as biomarkers of 1-BP exposure in humans.
Key Words: 1-bromopropane; protein adduct; globin; neurofilaments; mercapturate; biomarkers.
This paper was presented in part at the 46th Annual Society of Toxicology Meeting in Charlotte, NC, in March 2007.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. W. Hanley, M. R. Petersen, K. L. Cheever, and L. Luo N-Acetyl-S-(n-Propyl)-L-Cysteine in Urine from Workers Exposed to 1-Bromopropane in Foam Cushion Spray Adhesives Ann. Hyg., October 1, 2009; 53(7): 759 - 769. [Abstract] [Full Text] [PDF]
|
|