ToxSci Advance Access originally published online on July 26, 2007
Toxicological Sciences 2007 99(2):512-521; doi:10.1093/toxsci/kfm188
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Low and Nontoxic Inorganic Mercury Burdens Attenuate BCR-Mediated Signal Transduction
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* Department of Environmental Medicine, University of Rochester, Rochester, NY
Department of Immunology and Microbiology, Wayne State University, Detroit, MI
Department of Pharmacology, Wayne State University, Detroit, MI
1 To whom correspondence should be addressed at the Department of Immunology and Microbiology, 7374 Scott Hall, Wayne State University School of Medicine, Detroit, MI 48201, Fax: (313) 577-1155. E-mail: arosenspire{at}wayne.edu.
Received May 18, 2007; accepted July 18, 2007
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Abstract |
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The ubiquitous environmental heavy metal contaminant mercury (Hg) is a potent immunomodulator that has been implicated as a factor contributing to autoimmune disease. However, the mechanism(s) whereby Hg initiates or perpetuates autoimmune responses, especially at the biochemical/molecular level, remain poorly understood. Recent work has established a relationship between impaired B-cell receptor (BCR) signal strength and autoimmune disease. In previous studies, we have shown that in mouse WEHI-231 B cells, noncytotoxic concentrations of inorganic mercury (Hg+2) interfered with BCR-mediated growth control, suggesting that BCR signal strength was impaired by Hg+2. Extracellular signal-regulated kinase (ERK) 1,2 mitogen-activated protein kinase (MAPK) is responsible for the activation of several transcription factors in B cells. Phosphorylation of ERK serves as an essential node of signal integration for the BCR. Thus, the magnitude of ERK activation serves as an operational metric for BCR signal strength. Using Western blotting and phospho-specific flow cytometry, we now show that the kinetics and magnitude of BCR-mediated activation of ERK-MAPK are markedly attenuated in WEHI-231 cells and splenic B cells that have been exposed to low and nontoxic burdens of Hg+2. However, Hg+2 does not seem to act directly on ERK-MAPK but rather on an upstream element or elements of the BCR signal transduction pathway, above the level of the key protein tyrosine kinase Syk. Our data suggest that the site of action of Hg+2 may very well be localized on the plasma membrane. These findings support a connection between Hg+2 and attenuated BCR signal strength in the etiology of autoimmune disease.
Key Words: mercury; B-cell receptor; ERK; Syk.
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