Skip Navigation



ToxSci Advance Access published online on June 12, 2003
Toxicological Sciences, doi:10.1093/toxsci/kfg158
Toxicological Sciences © Society of Toxicology 2003; all rights reserved
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
75/1/82    most recent
kfg158v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Jia, X.
Right arrow Articles by Xiao, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jia, X.
Right arrow Articles by Xiao, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received March 25, 2003; accepted May 22, 2003
© 2003 Society of Toxicology

Risk Assessment

Compromised DNA Repair Enhances Sensitivity of the Yeast RNR3-lacZ Genotoxicity Testing System

Xuming Jia 1 and Wei Xiao 1*

1 Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK S7N 5E5 Canada

* To whom correspondence should be addressed. E-mail: wei.xiao{at}usask.ca.


  Abstract

The RNR3-lacZ genotoxicity testing system was developed based on the induction of a Saccharomyces cerevisiae RNR3-lacZ reporter gene in response to a broad range of DNA-damaging agents. In order to enhance the sensitivity of the RNR3-lacZ system, several deletion mutant strains representing different repair pathways were created and examined for their effects on RNR3-lacZ expression. It was found that inactivation of different DNA repair pathways has profound effects on the DNA damage induction of RNR3 expression. While deletion of MAG1 in the base excision repair pathway enhances the detection sensitivity to DNA alkylating agents, and deletion of RAD2 in the nucleotide excision repair pathway enhances the detection sensitivity to UV and agents that produce bulky lesions, inactivation of genes involved in the recombination repair and postreplication repair variably reduces RNR3-lacZ induction. This study not only helps to establish a more sensitive genotoxicity testing system, but also suggests that certain eukaryotic DNA repair pathways are required for gene regulation in response to DNA damage and probably serve as sensors in the signal transduction cascade.

Key Words: RNR3-lacZ, gene regulation, genotoxicity test, sensitivity, DNA repair, eukaryotes .


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
M. Zhang, Y. Liang, X. Zhang, Y. Xu, H. Dai, and W. Xiao
Deletion of Yeast CWP Genes Enhances Cell Permeability to Genotoxic Agents
Toxicol. Sci., May 1, 2008; 103(1): 68 - 76.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
K. Ichikawa and T. Eki
A Novel Yeast-Based Reporter Assay System for the Sensitive Detection of Genotoxic Agents Mediated by a DNA Damage-Inducible LexA-GAL4 Protein
J. Biochem., January 1, 2006; 139(1): 105 - 112.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.