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ToxSci Advance Access published online on June 12, 2003
Toxicological Sciences, doi:10.1093/toxsci/kfg161
Toxicological Sciences © Society of Toxicology 2003; all rights reserved
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Received March 10, 2003; accepted May 27, 2003
© 2003 Society of Toxicology

Reproductive and Developmental Toxicology

Proliferative Effects of Estradiol, Progesterone and Two CB-Congeners and Their Metabolites on Grey Seal (Halichoerus grypus) Uterine Myocytes in Vitro

B-M Bäcklin 1, C Bredhult 2, and M Olovsson 2*

1 Swedish Museum of Natural History, contamination research group, Box 50007, SE-104 05 Stockholm, Sweden
2 Department of Women's and Children's Health and The Centre for Reproductive Biology in Uppsala, Uppsala University, SE-751 85 Uppsala, Sweden

* To whom correspondence should be addressed. E-mail: matts.olovsson{at}kbh.uu.se.


  Abstract

Grey seal females living in the Baltic Sea have been found to exhibit a high prevalence of uterine leiomyomas. These animals are also known to accumulate lipid-soluble PCBs in their blubber. PCBs have documented endocrine disrupting effects, and to investigate whether the PCBs could be part of the genesis of uterine smooth muscle tumours in this species, grey seal myometrial cell cultures were exposed to two CBs and their metabolites, as well as to estradiol and progesterone, after which the effects were analysed in terms of proliferative activity by measurements of BrdU-absorbance and protein content. Progesterone was found to have an inhibitory effect, whereas one CB acted as a stimulant on the myometrial cell proliferation. One of the CB metabolites also seemed to have an inhibitory effect, which however could not be statistically verified. These results suggest that some CBs have effects on uterine myometrial cell proliferation in grey seals and may thus also take part in the growth regulation of uterine leiomyomas.

Key Words: Grey seal, CB, myometrium, proliferation, in vitro .


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Human and Experimental Toxicology, October 1, 2007; 26(10): 801 - 809.
[Abstract] [PDF]


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