Skip Navigation



ToxSci Advance Access published online on September 11, 2003
Toxicological Sciences, doi:10.1093/toxsci/kfg221
Toxicological Sciences © Society of Toxicology 2003; all rights reserved
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
76/1/83    most recent
kfg221v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Chen, J.-H.
Right arrow Articles by Wang, C.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J.-H.
Right arrow Articles by Wang, C.-J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received June 6, 2003; accepted August 11, 2003
© 2003 Society of Toxicology

Environmental Toxicology

Gaseous Nitrogen Oxides Stimulate Cell Cycle Progression by Rb Phosphorylation via Activation of Cyclins/Cdks

Jing-Hsien Chen 1, Tsui-Hwa Tseng 2, Yung-Chyan Ho 2, Hui-Hsuan Lin 1, Wea-Lung Lin 3, and Chau-Jong Wang 1*

1 Institute of Biochemistry, Chung Shan Medical University, Taichung, Taiwan
2 Department of Applied Chemistry, Chung Shan Medical University, Taichung, Taiwan
3 Department of Pathology, Chung Shan Medical University Hospital, Taichung, Taiwan

* To whom correspondence should be addressed. E-mail: wcj{at}csmu.edu.tw.


  Abstract

Nitrogen oxides (NOx) are important indoor and outdoor air pollutants. Many studies have indicated that NOx gas causes lung tissue damage by its oxidation properties and its free-radicals. In a previous study we demonstrated that NOx gas induced proliferation of human lung fibroblast MRC-5 cells. In this study we show that NOx gas stimulates MRC-5 cell proliferation by Rb phosphorylation via activation of cyclin-cdk complexes. Western blot and immunoprecipitation data showed that NOx gas increased the expressions of cyclinA/cdk2, cyclinD1/cdk4 and cyclinE/cdk2 complexes in the cells 9 h after treatment. The levels of phospho-Rb were also increased and cdk inhibitors (CKIs) p27 and p16 were apparently decreased. These data suggested that NOx gas stimulates cell cycle progression by Rb phosphorylation via activation of cyclin-cdk complexes and inhibition of CKIs. In conclusion, the NOx gas induced lung fibroblast cell proliferation by stimulation of cell cycle progression may contribute to lung fibrosis by NOx pollutants.

Key Words: Gaseous nitrogen oxides, proliferation, cdk inhibitor(CKI), Rb phosphorylation, cell cycle progression .


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
J.-H. Chen, H.-H. Lin, T.-A. Chiang, J.-D. Hsu, H.-H. Ho, Y.-C. Lee, and C.-J. Wang
Gaseous Nitrogen Oxide Promotes Human Lung Cancer Cell Line A549 Migration, Invasion, and Metastasis via iNOS-Mediated MMP-2 Production
Toxicol. Sci., December 1, 2008; 106(2): 364 - 375.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.