ToxSci Advance Access published online on November 4, 2003
Toxicological Sciences, doi:10.1093/toxsci/kfh006
Toxicological Sciences © Society of Toxicology 2003; all rights reserved
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1 Laboratoire de Pharmacologie-Toxicologie, Institut National de Recherche Agronomique, 31931 Toulouse, France
* To whom correspondence should be addressed. E-mail: ioswald{at}toulouse.inra.fr.
Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium verticillioides (formerly F. moniliforme), a fungus that commonly contaminates maize. FB1 causes toxicological effects in laboratory and domestic animals including pigs. As the gastrointestinal tract represents the first barrier met by exogenous food compounds, the purpose of this study was to investigate the effects of FB1 on IPEC-1, a porcine intestinal epithelial cell line. We first verified that low concentrations of FB1 did not exert any cytotoxic effect on IPEC-1. Indeed significant LDH release was only observed for FB1 concentrations greater than 50 and 700 µM on proliferating and non-proliferating cells respectively. We then demonstrated that FB1 inhibits proliferation of IPEC-1. FACS analysis of the cell cycle indicated that FB1 blocks the proliferation of intestinal cells in the G0/G1 phase. Similar results were obtained with LLC-PK1, a renal porcine epithelial cell line, which is considered to be a good model for studying FB1 in vitro effects. We have also assessed the effects of FB1 on the integrity of the barrier formed by the intestinal epithelium. We demonstrated that FB1 decreases the trans-epithelial electrical resistance of IPEC-1 in a time- and dose-dependent manner. This effect was only noticed after a long exposure (8-12 days of treatment). FB1 induced the TEER decrease independently of the cell differentiation stage and this effect was partially reversible. Taken together, our data indicate that FB1 alters the proliferation and the barrier function of intestinal cells. These results may have implications for humans and animals consuming FB1 contaminated food or feed.
© 2003 Society of Toxicology
Systems Toxicology
The Mycotoxin Fumonisin B1 Alters the Proliferation and the Barrier Function of Porcine Intestinal Epithelial Cells
2 Instituto Nazionale di Ricerca per gli Alimenti e la Nutrizione, 00178 Roma, Italy
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