Skip Navigation



ToxSci Advance Access published online on December 22, 2003
Toxicological Sciences, doi:10.1093/toxsci/kfh052
Toxicological Sciences © Society of Toxicology 2003; all rights reserved
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
78/1/15    most recent
kfh052v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Viau, C.
Right arrow Articles by Charbonneau, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Viau, C.
Right arrow Articles by Charbonneau, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received August 14, 2003; accepted November 30, 2003
© 2003 Society of Toxicology

Biotransformation and Toxicokinetics

Dietary Fibers Reduce the Urinary Excretion of 1-Hydroxypyrene Following Intravenous Administration of Pyrene

C. Viau 1*, C. Zaoui 1, and S. Charbonneau 1

1 Human Toxicology Research Group, Department of Environmental and Occupational Health, University of Montreal, P.O. Box 6128, Main Station, Montreal (QC) Canada, H3C 3J7

* To whom correspondence should be addressed. E-mail: claude.viau{at}umontreal.ca.


  Abstract

During biological monitoring of exposure to a chemical, a possible source of inter-individual variability in the measurement of a urinary metabolite that undergoes enterohepatic cycling is the presence of dietary fiber in the gastro-intestinal tract. This study examined the effect of diets containing either the insoluble fiber Alphacel (non-nutritive bulk cellulose) or the soluble pectin (from citrus fruit, MW 20,000-40,000). Five groups of male Sprague-Dawley rats received one of the following diets: poor (5% w/w) or rich (15% w/w) in Alphacel, poor (5% w/w) or rich (15% w/w) in pectin or no fiber (NF). Five µmol/kg of pyrene was administered by iv injection immediately after feeding the animals with their respective diet, and urine and feces collections started for the determination of 1-hydroxypyrene (1-OHP), a metabolite of pyrene. The type of fiber had no influence on the results. Both the rats receiving diets poor and rich in fiber excreted less 1-OHP (18 ± 8 and 15 ± 7 pmol per g of rat, respectively) in the 24-h urine samples than the NF group (28 ± 6 pmol/g). There was a non-statistically significant trend towards increased fecal and total [urinary + fecal] 1-OHP excretion with increasing amount of fiber in the diet. An in vitro experiment showed an inverse correlation (R2 = 0.98) between the amount of Alphacel in suspension in a 1-OHP aqueous solution and the recovery of 1-OHP from the soluble fraction. The reduction in urinary output of the metabolite due to fiber reaching ca. 40% may contribute to its interindividual variability observed in occupational and environmental studies.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.