Skip Navigation



ToxSci Advance Access published online on May 24, 2004
Toxicological Sciences, doi:10.1093/toxsci/kfh173
Toxicological Sciences © Society of Toxicology 2004; all rights reserved
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
81/1/103    most recent
kfh173v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Cheng, Y. W.
Right arrow Articles by Kang, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, Y. W.
Right arrow Articles by Kang, J. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received March 2, 2004
Accepted May 7, 2004

Genetic Toxicology

Genotoxicity of Motorcycle-Exhaust Particulate in Vivo and in Vitro

Yu Wen Cheng 1, Wen Wha Lee 2, Ching Hao Li 2, Chen Chen Lee 2, Jaw Jou Kang 2*

1 School of Pharmacy, Taipei Medical University, Taipei, Taiwan
2 Institute of Toxicology, College of Medicine, National Taiwan University, Taipei, Taiwan

* To whom correspondence should be addressed. E-mail: jjkang{at}ha.mc.ntu.edu.tw.


  Abstract

The genotoxic potency of motorcycle exhaust particles (MEP) was investigated by use of a bacterial reversion assay, and chromosome aberration and micronucleus test in this study. In the bacterial reversion assay (Ames test), MEP concentration-dependently increased TA98, TA100, and TA102 revertants in the presence of metabolic activating enzymes. In the chromosome aberration test, MEP concentration-dependently increased abnormal structural chromosomes in CHO-K1 cells both with and without S-9. Pretreatment with antioxidants ({alpha}-tocopherol, ascorbate, catalase, and NAC) showed varying degrees of inhibitory effect on the MEP-induced mutagenic effect and chromosome structural abnormalities. In the in vivo micronucleus test, MEP dose-dependently induced micronucleus formation in peripheral red blood cells after 24 and 48 h of treatment. The increase of micronucleated reticulocytes induced by MEP can be inhibited by pretreatment with {alpha}-tocopherol and ascorbate. The fluorescence intensity of DCFH-DA loaded CHO-K1 cells were increased upon addition of MEP. Our data suggest that MEP can induce genotoxicity through a reactive oxygen species (ROS)-dependent pathway, which can be augmented by metabolic activation. {alpha}-Tocopherol, ascorbate, catalase, and NAC can inhibit MEP-induced genotoxicity, indicating that reactive oxygen species might be involved in this effect.

Key Words: Motorcycle exhaust particles, Ames test, reactive oxygen species, chromosome aberration, micronucleus, genotoxicity .


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J R Soc InterfaceHome page
L. Muller, M. Riediker, P. Wick, M. Mohr, P. Gehr, and B. Rothen-Rutishauser
Oxidative stress and inflammation response after nanoparticle exposure: differences between human lung cell monocultures and an advanced three-dimensional model of the human epithelial airways
J R Soc Interface, July 8, 2009; (2009) rsif.2009.0161.focusv1.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.