ToxSci Advance Access published online on July 28, 2004
Toxicological Sciences, doi:10.1093/toxsci/kfh235
Toxicological Sciences © Society of Toxicology 2004; all rights reserved
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1 Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, Wisconsin 53705
* To whom correspondence should be addressed. E-mail: repeterson{at}pharmacy.wisc.edu.
ZfAHR2 has been identified as the receptor that is essential for mediating the developmental toxicity caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in zebrafish. One form of zfARNT2, zfARNT2b, forms a functional heterodimer with zfAHR2 that specifically recognizes XREs in gel shift experiments and induces XRE-driven transcription in COS-7 cells treated with TCDD. However, it has not been demonstrated that zfARNT2b acts as the physiological dimerization partner for zfAHR2 to mediate TCDD toxicity in developing zebrafish. An antisense morpholino targeted against zfARNT2 (zfarnt2-MO) along with a line of mutant zebrafish lacking expression of the zfarnt2 gene have been used to test the hypothesis that zfARNT2 mediates the developmental toxicity of TCDD. Injection of the zfarnt2-MO decreased expression of the zfARNT2 protein but did not provide any protection against the formation of pericardial edema at 72 hpf. In addition, in TCDD dose response studies the zfarnt2-/- embryos showed no protection against three endpoints of TCDD toxicity observed at 96 hpf: pericardial edema, reduced trunk blood flow, and shortened lower jaw. Finally, immunostaining results at 96 hpf demonstrate that the zfarnt2-/- embryos show a similar pattern of TCDD-induced zfCYP1A expression as WT embryos These results demonstrate that zfARNT2 is not essential for mediating TCDD developmental toxicity in zebrafish and suggest that alternate dimerization partner(s) exist for zfAHR2 in vivo.
Accepted July 26, 2004
Reproductive and Developmental Toxicology
ARNT2 Is Not Required for TCDD Developmental Toxicity in Zebrafish
2 School of Pharmacy, University of Wisconsin, Madison, Wisconsin 53705; Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, Wisconsin 53705
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