Skip Navigation



ToxSci Advance Access published online on December 22, 2004
Toxicological Sciences, doi:10.1093/toxsci/kfi068
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
85/1/502    most recent
kfi068v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lin, G.-f.
Right arrow Articles by Shen, J.-h.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, G.-f.
Right arrow Articles by Shen, J.-h.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Toxicological Sciences © Society of Toxicology 2004; all rights reserved.
Received November 11, 2004
Accepted December 17, 2004

Carcinogenicity

An Association of UDP-Glucuronosyltransferase 2B7 C802T (His268Tyr) Polymorphism with Bladder Cancer in Benzidine-Exposed Workers in China

Guo-fang Lin 1, Wei-chao Guo 1, Ji-gang Chen 2, Yi-qing Qin 2, Klaus Golka 3, Cui-qing Xiang 2, Qing-wen Ma 1, Da-ru Lu 4, and Jian-hua Shen 1*

1 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
2 Municipal Center for Disease Prevention and Control, Shanghai 200236, China
3 Institute for Occupational Physiology at the University of Dortmund, 44139 Dortmund, Germany
4 Institute of Genetics, Fudan University, Shanghai 200333, China

* To whom correspondence should be addressed.
Jian-hua Shen, E-mail: jhshen{at}sibs.ac.cn


  Abstract

UDP-Glucuronyltransferase 2B7 (UGT2B7) is involved in benzidine metabolism, as demonstrated by in vitro experiments with liver slices. To evaluate the possible association of UGT2B7 gene polymorphism with bladder cancer risk for benzidine-exposed subjects, diagnosed bladder cancer cases (n=36) who were members of a cohort of benzidine exposed workers in the Chinese dyestuff industry were investigated. UGT2B7 polymorphism at locus C802T (His268Tyr ) was detected using a PCR-RFLP based procedure. Non-diseased cohort members (156 men, 95 women) were taken as work-related control and unexposed healthy individuals (113 men, 105 women) were taken as community control. The data showed that the polymorphism at locus UGT2B7 C802T in a general Chinese population significantly differs from that in a Caucasian population (p=0.00018) displaying a distinctly lower frequency of T/T genotypes (9.2% vs. 25.3%), while no significant difference to a Japanese population could be detected (p=0.17). A higher prevalence of T/T genotype carriers was found in the cancer cases, compared with unexposed healthy controls (25% vs. 9%, OR 3.30, 95% CI 1.37-7.98, p=0.006). A higher presentation of T allele carriers in the patients group was also confirmed (46% vs. 33%, OR 1.73, 95% CI 1.05-2.87, p=0.03). A higher portion of the T/T genotype was also observed in bladder cancer patients compared with non-diseased members of the same benzidine-exposed cohort although some of them displayed different degrees of cellular alterations in their exfoliated urothelial cells. This study points for the first time to an association between a homozygous mutant genotype of human UDP-glucuronosyltransferase 2B7 catalyzing the biotransformation of benzidine and an elevated bladder cancer risk for formerly benzidine-exposed workers of the dyestuff industry.

Keywords: Aromatic amines; Glucuronidation; Transitional cell carcinoma; Chinese.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.