Skip Navigation



ToxSci Advance Access published online on July 13, 2005
Toxicological Sciences, doi:10.1093/toxsci/kfi256
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
88/2/412    most recent
kfi256v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Braydich-Stolle, L.
Right arrow Articles by Hofmann, M.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braydich-Stolle, L.
Right arrow Articles by Hofmann, M.-C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received May 13, 2005
Accepted June 28, 2005

In Vitro Toxicology

IN VITRO CYTOTOXICITY OF NANOPARTICLES IN MAMMALIAN GERM-LINE STEM CELLS

Laura Braydich-Stolle 1, Saber Hussain 2, John Schlager 3, and Marie-Claude Hofmann 1*

1 Department of Biology, The University of Dayton, 300 College Park, Dayton, OH 45469
2 Alion Science & Technology, Dayton, OH 45437
3 Operational Toxicology Branch, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH 45433

* To whom correspondence should be addressed.
Marie-Claude Hofmann, E-mail: Marie-Claude.Hofmann{at}notes.udayton.edu


  Abstract

Gametogenesis is a complex biological process that is particularly sensitive to environmental insults such as chemicals. Many chemicals have a negative impact on the germ line, either by directly affecting the germ cells, or indirectly through their action on the somatic nursing cells. Ultimately, these effects can inhibit fertility, and may have negative consequences on the development of the offspring. Recently, nanomaterials such as nanotubes, nanowires, fullerene derivatives (buckyballs) and quantum dots have received enormous national attention to create new types of analytical tools for biotechnology and the life sciences. Despite the wide application of nanomaterials, there is a serious lack of information concerning their impact on human health and the environment. Thus, there are limited studies available on toxicity of nanoparticles for risk assessment of nanomaterials. The purpose of this study was to assess the suitability of a mouse spermatogonial stem cell line as a model to assess nanotoxicity in the male germ line in vitro. The effects of different types of nanoparticles on these cells was evaluated using light microscopy, cell proliferation and standard cytotoxicity assays. Our results demonstrate a concentration-dependent toxicity for all types of particles tested, while the corresponding soluble salts had no significant effect. Ag nanoparticles were the most toxic while MoO3 nanoparticles were the least toxic. Our results suggest that this cell line provides a valuable model to assess the cytotoxicity of nanoparticles in the germ line in vitro.

Keywords: nanoparticles; toxicity; cell line; spermatogonia; stem cells.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
S. M. Hussain and J. J. Schlager
Safety Evaluation of Silver Nanoparticles: Inhalation Model for Chronic Exposure
Toxicol. Sci., April 1, 2009; 108(2): 223 - 224.
[Full Text] [PDF]

Home page
 International Journal of ToxicologyHome page
J. E. Skebo, C. M. Grabinski, A. M. Schrand, J. J. Schlager, and S. M. Hussain
Assessment of Metal Nanoparticle Agglomeration, Uptake, and Interaction Using High-Illuminating System
International Journal of Toxicology, March 1, 2007; 26(2): 135 - 141.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
J. G. Teeguarden, P. M. Hinderliter, G. Orr, B. D. Thrall, and J. G. Pounds
Particokinetics In Vitro: Dosimetry Considerations for In Vitro Nanoparticle Toxicity Assessments
Toxicol. Sci., February 1, 2007; 95(2): 300 - 312.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
S. M. Hussain, A. K. Javorina, A. M. Schrand, H. M. Duhart, S. F. Ali, and J. J. Schlager
The Interaction of Manganese Nanoparticles with PC-12 Cells Induces Dopamine Depletion
Toxicol. Sci., August 1, 2006; 92(2): 456 - 463.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.