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ToxSci Advance Access published online on August 24, 2005
Toxicological Sciences, doi:10.1093/toxsci/kfi296
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© The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received July 7, 2005
Accepted August 18, 2005

Reproductive and Developmental Toxicology

Late Gestation Rat Myometrial Cells Express Multiple Isoforms of Phospholipase A2 that Mediate PCB 50-induced Release of Arachidonic Acid with Coincident Prostaglandin Production

Kelly Brant 1* and Rita Loch Caruso 1

1 Department of Environmental Health Sciences, University of Michigan, Ann Arbor, MI USA

* To whom correspondence should be addressed.
Kelly Brant, E-mail: kbrant{at}umich.edu


  Abstract

Previous reports have shown that ortho-substituted polychlorinated biphenyls (PCBs) are uterotonic and activate phospholipase A2 to release arachidonic acid (AA) from membrane phospholipids. AA serves as the precursor to various eicosanoids, which, in addition to AA itself, are capable of modulating uterine function. To examine whether PCBs stimulate PLA2 to mobilize arachidonic acid from late gestation rat uterus, primary cultures of gestation day (gd) 20 rat myometrial cells (RMC) were labeled with 0.5 µCi [3H]-AA prior to a 10, 20 or 30 min exposure to PCB 50 (1-50 µM) or 0.1 % DMSO (solvent control). PCB 50 stimulated the release of [3H]-AA from gd20 RMC in concentration- and time- dependent manners (p<0.05). PCB 50 stimulation of RMC was attenuated with EGTA and nifedipine, suggesting that AA release was dependent on the influx of extracellular calcium through L-type voltage operated calcium channels. PCB 50-induced release of AA from RMC was also attenuated with the PLA2-specific inhibitors MAFP, BEL and manoalide (p<0.05). Stimulation of PLA2 enzymes in response to PCB exposure occurred via p38 MAPK activation as indicated by the significant attenuation of PCB 50-induced AA release from RMC in the presence of SB 202190. In addition to stimulating AA release, PCB 50 induced a significant production of prostaglandins from gd20 RMC compared with controls (p<0.05). These results suggest that myometrial cells express multiple PLA2 isoforms that may serve as a target and effector for ortho-substituted PCB-mediated stimulation of uterine function through arachidonic acid and prostaglandin release.

Keywords: myometrium; PCBs; arachidonic acid; prostaglandins; Phospholipase A2 .
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