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ToxSci Advance Access published online on September 21, 2005
Toxicological Sciences, doi:10.1093/toxsci/kfi333
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© The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received August 22, 2005
Accepted September 15, 2005

Reproductive and Developmental Toxicology

Protective Effect of Quercetin on Aroclor 1254-Induced Oxidative Damage in Cultured Chicken Spermatogonial Cells

Yuling Mi 1 and Caiqiao Zhang 1*

1 Department of Veterinary Medicine, College of Animal Sciences Zhejiang University, Hangzhou 310029, China

* To whom correspondence should be addressed.
Caiqiao Zhang, E-mail: cqzhang{at}zju.edu.cn


  Abstract

Quercetin, a dietary-derived falvonol-type flavonoid, is ubiquitous in fruits and vegetables and plays important roles in human health due to its antioxidant function. The present study was performed to investigate effects of quercetin on oxidative damage that was induced by an environmental endocrine disrupter, Aroclor 1254 (A1254), in cultured spermatogonial cells of embryonic chickens. Spermatogonial cells were dispersed from 18-day-old embryo and exposed to A1254 alone or in combinations with quercetin. The oxidative damage was estimated by measuring contents of thiobarbituric acid reactive substances (TBARS, an indicator of lipid peroxidation), activity of superoxide dismutase (SOD, a scavenger of superoxide) and glutathione (GSH, an intracellular antioxidant). Results showed that quercetin had no deleterious effect on spermatogonial cells at 0.01~1µg/ml. Exposure to A1254 (10µg/ml) induced an increase of spermatogonial cell number and the membrane integrity was damaged by elevation of lactate dehydrogenase (LDH) leakage. Exposure to A1254 also induced an elevation of TBARS but a decrease in SOD activity and GSH content. However, compared with A1254 treatment alone, simultaneous supplementation with quercetin decreased LDH leakage to maintain the cell integrity, decreased the levels of TBARS to quench the free radicals, increased SOD activity and GSH content to restore endogenous antioxidant defense system. Thus, quercetin displayed protective effects on spermatogonial cells from A1254-induced oxidative damage through increasing intracellular antioxidant level and decreasing lipid peroxidation. Consequently, the antioxidant from food or feed consumed by human and animals, such as quercetin, may attenuate negative effects of environmental endocrine disrupters.

Keywords: Quercetin; Polychlorinated biphenyls; Oxidative damage; Chicken; Spermatogonial cells.
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