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ToxSci Advance Access published online on November 16, 2005
Toxicological Sciences, doi:10.1093/toxsci/kfj042
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© The Author 2005. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received August 31, 2005
Accepted October 13, 2005

Endocrine Toxicology

AhR-Agonist-Induced Transcriptional Changes of Genes Involved in Thyroid Function in Primary Porcine Thyrocytes

Pocar P 1 *, Klonisch T 2, Brandsch C 3, Eder K 3, Fröhlich C 1, Hoang-Vu C 4, and S Hombach-Klonisch 2

1 Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany
2 Department of Human Anatomy and Cell Science, Faculty of Medicine, University of Manitoba, Winnipeg (MB) R3E0W3 Canada
3 Institute of Nutritional Sciences, Agricultural Faculty, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany
4 Clinics of Surgery, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

* To whom correspondence should be addressed.
Pocar P, E-mail: paola.pocar{at}unimi.it


  Abstract

The Ah receptor (AhR) is a ligand transcription factor mediating toxic effects of chemicals such as dioxins. The 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) and the coplanar polychlorinated biphenyl 126 (PCB 126) are member of the polyhalogenated aromatic hydrocarbons family exerting a variety of toxic effects in tissue- and specie-specific manner including thyroid function. In the present study, we aimed to investigate the effects of TCDD (1 and 10 nM) and dioxin-like PCB 126 (306 nM) on AhR signaling pathway and on the gene expression profiles of key factors involved in thyroid function, including thyroglobulin (TG), thyroid peroxidase (TPO), sodium iodide symporter (NIS), TSH receptor (TSHR) and cathepsins (Cat B and L), using a primary porcine thyrocyte culture as experimental model. AhR and ARNT expression was detected both as mRNA and on the protein level. Expression did not vary upon treatment with either TCDD or PCB 126. However, treatment with TCDD and PCB 126 induced an AhR signaling response as indicated by the expression of the AhR-target gene cytochrome p450 1A1 (CYP1A1). Both 10 nM TCDD and PCB 126 treatment induce a significant down-regulation in the expression of NIS and cathepsin B without affecting any of the other parameters investigated. In conclusion, these data indicate that (a) thyrocytes are targets of TCDD and TCDD-like compounds and (b) there is evidence for two independent most likely AhR-mediated molecular mechanisms, by which these compounds negatively interfere with thyroid function.

Keywords: tetrachlorodibenzo-p-dioxin (TCDD); polychlorinated biphenyls (PCBs); thyroid; porcine; cathepsin B; sodium iodide symporter (NIS).

Grant support: This work was supported by the Wilhelm Roux Program of the Martin Luther University Halle/Wittenberg.


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