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ToxSci Advance Access published online on November 13, 2006
Toxicological Sciences, doi:10.1093/toxsci/kfl166
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© The Author 2006. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received September 15, 2006
Accepted October 17, 2006

Genetic Toxicology

OGG1 and MYH are Involved in the Incision of Arsenite-Induced DNA Adducts

Yeong-Shiau Pu 1, Kun-Yan Jan 2, Tsing-Cheng Wang 2, Alexander S. S. Wang 1, and Jia-Ran Gurr 3 *

1 Department of Urology, National Taiwan University College of Medicine, Taipei, Taiwan, ROC
2 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, Taiwan, ROC
3 Hsing Wu College, No. 101, Sec. 1, Fen-Liao Rd., Linkou, Taipei County 24452, Taiwan, ROC

* To whom correspondence should be addressed.
Jia-Ran Gurr, E-mail: 090012{at}mail.hwc.edu.tw


  Abstract

Since arsenite is known to induce oxidative DNA damage in human cells, we asked if it induces other types of DNA damage and how the DNA damage is repaired. Treatment of human promyelocytic leukemia NB4 cells with 0.5 µM As2O3 for 30 min induced no DNA breaks, as analyzed by a standard comet assay. However, breaks were detected if these cells were then digested with endonuclease III (EnIII), formamidopyrimidine-DNA glycosylase (Fpg), or a nuclear extract (NE) of NB4 cells. Using either H2O2-Fe treated nuclei or As2O3-treated cells, digestion with either NE or EnIII+Fpg generated the same amount of breaks, and subsequent treatment with EnIII+Fpg resulted in no increase in breaks in NE-digested cells and vice versa. The human cell lines, defective in nucleotide excision protein, such as XPA, XPD, and XPG, excised UVC-induced adducts less rapidly than normal fibroblasts, but excised As2O3-adducts at the same rate as the normal cells. Immunodepletion of the NE with antibody against OGG1 or MYH decreased the incision of As2O3-induced adducts, while antibodies against XPA, XPB, XPD, XPF, or XPG, did not. These results suggest that As2O3 induces the formation of only oxidative DNA adducts and that OGG1 and MYH are involved in this incision process.

Keywords: Arsenic trioxide; Oxidative damage; Monomethylarsonic acid; Dimethylarsinic acid; Sodium arsenite.
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