Skip Navigation



ToxSci Advance Access published online on June 16, 2007
Toxicological Sciences, doi:10.1093/toxsci/kfm164
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
99/1/174    most recent
kfm164v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lilja, J.
Right arrow Articles by Forsby, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lilja, J.
Right arrow Articles by Forsby, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Surfactant-Induced TRPV1 Activity- a Novel Mechanism for Eye Irritation?

Johanna Lilja*, Heléne Lindegren and Anna Forsby

Department of Neurochemistry, Stockholm University, Svante Arrhenius v. 21 A, SE-106 91, Stockholm, Sweden

* Corresponding author: Johanna Lilja, Phone:0046(8)164269, Fax:0046(8)161371, email: johanna{at}neurochem.su.se

Received March 13, 2007; revision received May 31, 2007; accepted June 11, 2007


  Abstract

The pain receptor transient receptor potential vanilloid type 1 (TRPV1) has been reported as one of the key components in the pain pathway. Activation of the receptor causes a Ca2+ influx with secondary effects leading to neurogenic inflammation. Here we report specific activation of TRPV1 by detergent-containing hygiene products measured as intracellular Ca2+ influxes in stably TRPV1-expressing neuroblastoma SH-SY5Y cells. Children products marketed as "painless" (containing lower concentration of detergents), and conditioners (without detergents) did not induce specific TRPV1 activation. Furthermore, low concentrations of the detergent sodium lauryl sulfate (SLS) dose-dependently induced Ca2+ influxes that could be addressed to TRPV1. These results reveal a novel mechanistic pathway for surfactant-induced nociception, which may be an important endpoint in in vitro test batteries as alternatives to Draize's rabbit eye test for classification of eye irritating products.

Key Words: pain; detergent; TRPV1; eye; in vitro; SH-SY5Y.

Heléne Lindegren (helene{at}neurochem.su.se), Anna Forsby (annaf{at}neurochem.su.se)


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.