Skip Navigation



ToxSci Advance Access published online on December 7, 2007
Toxicological Sciences, doi:10.1093/toxsci/kfm296
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
102/1/82    most recent
kfm296v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Miyawaki, J.
Right arrow Articles by Masuno, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyawaki, J.
Right arrow Articles by Masuno, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

4-tert-Octylphenol Regulates the Differentiation of C3H10T1/2 Cells into Osteoblast and Adipocyte Lineages

Joji Miyawaki*,{dagger}, Setsuya Kamei*,{dagger}, Kenshi Sakayama*, Haruyasu Yamamoto* and Hiroshi Masuno{dagger},1

* Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Toon, Ehime 791-0295, Japan {dagger} Department of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Takooda, Tobe-cho, Iyo-gun, Ehime 791-2101, Japan

1 Address correspondence to: Hiroshi Masuno, Ph.D. Department of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Takooda, Tobe-cho, Iyo-gun, Ehime 791-2101, Japan Phone No. +81-89-958-2111 FAX No. +81-89-958-2177 e-mail address: hmasuno{at}epu.ac.jp

Received September 28, 2007; revision received November 22, 2007; accepted December 5, 2007


  Abstract

The aim of this study was to investigate whether 4-tert-octylphenol (OP) affects the differentiation of multipotent C3H10T1/2 cells, a cell line established from mouse embryonic connective tissue, into osteoblast and adipocyte lineages. Confluent C3H10T1/2 cells were incubated for seven days with (OP-treated cultures) or without (control cultures) 15 µg/ml of OP. The seven-day treatment of confluent cells with OP decreased alkaline phosphatase (ALP) activity by 81%, inhibited the expression of transforming growth factor β2 (TGFβ2), and inhibited the morphological changes in cells to an osteoblastic appearance. These results indicate that the seven-day treatment of confluent C3H10T1/2 cells with OP inhibited their differentiation into osteoblasts. Since this treatment strongly induced the expression of peroxisome proliferator-activated receptor r (PPARr), but did not stimulate triacylglycerol (TG) accumulation in cells, C3H10T1/2 cells in the control and OP-treated cultures were incubated for two days with a hormone mixture (insulin, dexamethasone, and 1-methyl-3-isobutylxanthine) and incubated for an additional five days with insulin alone. The TG and adiponectin contents of the OP-treated cultures were 4.2 and 4.1 times higher, respectively, than those of the control cultures. There were many more Oil Red O-staining cells in the OP-treated cultures than in the control cultures. The expression of PPARr in the OP-treated cultures was higher than that in the control cultures. These results indicate that the OP-treated cultures contained a larger number of adipocytes than the control cultures. In conclusion, treatment of C3H10T1/2 cells with OP inhibited osteoblast differentiation, causing a lineage shift towards adipocytes.

Key Words: 4-tert-octylphenol; C3H10T1/2 cells; osteoblast differentiation; adipocyte differentiation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.