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ToxSci Advance Access published online on January 27, 2008
Toxicological Sciences, doi:10.1093/toxsci/kfn014
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© The Author 2008. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org.

Cellular response to diesel exhaust particles strongly depends on the exposure method

Amara L. Holder*, Donald Lucas{dagger}, Regine Goth-Goldstein{dagger} and Catherine P. Koshland{ddagger}

* 1 Cyclotron Rd MS 70-108B, Berkeley, CA, 94720-8168, Ph: 510-486-4134, Fax: 510-486-7303 (aholder{at}lbl.gov) {dagger} Environmental Energy Technologies Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-8168 (D_Lucas{at}lbl.gov, R_Goth-Goldstein{at}lbl.gov) {ddagger} Division of Environmental Health Sciences, School of Public Health, University of California Berkeley, Berkeley, CA 94720-7360 (ckosh{at}berkeley.edu)

Corresponding Author: Amara Holder, Email: aholder{at}lbl.gov

Received November 2, 2007; revision received January 12, 2008; accepted January 16, 2008


  Abstract

In vitro exposure to aerosols at the air-liquid interface (ALI) preserves the physical and chemical characteristics of aerosol particles. Although frequently described as being a more physiologic exposure method, ALI exposure has not been directly compared with conventional in vitro exposures where the particles are suspended in medium. We exposed immortalized human bronchial epithelial cells (16HBE14o) to aerosolized diesel exhaust particles at the ALI and to suspensions of collected particles. The response of the cells was determined from measurements of the cell viability and Interleukin-8 (IL8) secretion. The deposited size distribution at the cell surface was measured with transmission electron microscopy to obtain a dose for the ALI exposure. While exposure by either method caused a slight decrease in cell viability and induced IL-8 secretion, the response to ALI exposure occurred at doses several orders of magnitude lower than exposure to particles in suspension. The most likely sources for the different dose responses are the artifacts introduced during the collection and resuspension of particles for conventional suspension exposures. The number concentration of particles deposited at the ALI is similar to the modeled deposition in the tracheal-bronchial (TB) region in a human lung, but the ALI size distribution is skewed towards particles larger than those deposited in the lung.

Key Words: Air-liquid interface; particle deposition; ultrafine particles; nanoparticle toxicology.


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