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ToxSci Advance Access published online on February 27, 2008
Toxicological Sciences, doi:10.1093/toxsci/kfn035
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© The Author 2008. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Influence of TCDD on zebrafish CYP1B1 transcription during development

Hou-Chu Yin*,{dagger}, Hua-Pin Tseng*, Hsin-Yu Chung*, Chin-Yi Ko*, Wen-Shyong Tzou*, Donald R. Buhler{ddagger} and Chin-Hwa Hu*,§

* Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan {dagger} Department of Medical Technology, Chung Hwa University of Medical Technology, Tainan, Taiwan {ddagger} Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon, U.S.A § Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan

Received September 29, 2007; revision received February 5, 2008; accepted February 6, 2008


  Abstract

Cytochrome P450 1B1 (CYP1B1) is a heme-containing monooxygenase that metabolizes various polycyclic aromatic hydrocarbons (PAHs) and arylamines, as well as retinoic acid and steroid hormones. Here we report the cloning of an ortholog of CYP1B1 from zebrafish and the demonstration that transcription of zebrafish CYP1B1 was modulated by two types of mechanisms during different developmental stage. First in late pharyngula stage before hatching, CYP1B1 was constitutively transcribed in retina, midbrain-hindbrain boundary (MHB) and diencephalon regions through a close coordination between AHR2-dependent and AHR2-independent pathways. After hatching, the basal transcription was attenuated and it could not be elicited upon TCDD exposure. In contrast, TCDD exposure induced de novo CYP1B1 transcription in larval branchial arches and heart tissues via an AHR2-dependent pathway. Blocking AHR2 translation completely eliminated the TCDD-mediated CYP1B1 transcription. However, we did not detect any types of CYP1B1 transcription in liver and kidney tissues through the developmental stage. It suggests that the constitutive and TCDD-inducible types of CYP1B1 transcriptions are modulated by distinct pathways with different tissue specificities. Finally, we investigated the role of CYP1B1 in TCDD-mediated embryonic toxicity. Since knockdown of CYP1B1 did not prevent TCDD-induced pericardial edema and cranial defects, it suggests that CYP1B1 is not involved in the developmental toxicity of dioxin.

Key Words: CYP1B1; transcription; zebrafish; embryo; larva; AHR2; TCDD.


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