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ToxSci Advance Access published online on January 29, 2009
Toxicological Sciences, doi:10.1093/toxsci/kfp020
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© The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

ANIT-induced intrahepatic cholestasis alters hepatobiliary transporter expression via Nrf2 dependent and independent signaling

Yuji Tanaka, Lauren M. Aleksunes, Yue Julia Cui and Curtis D. Klaassen*

Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, Kansas City, Kansas 66160, USA

* To whom correspondence should be addressed at: Department of Pharmacology, Toxicology, and Therapeutics, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160-7417 U.S.A. Phone: 913-588-7500 FAX: 913-588-7501, E-mail address: cklaasse{at}kumc.edu (C.D. Klaassen)

Received November 26, 2008; revision received January 22, 2009; accepted January 24, 2009


  Abstract

Alpha-naphthylisothiocyanate (ANIT) causes intrahepatic cholestasis by injuring biliary epithelial cells. Adaptive regulation of hepatobiliary transporter expression has been proposed to reduce liver injury during cholestasis. Recently, the oxidative stress transcription factor Nrf2 was shown to regulate expression of hepatobiliary transporters. The purpose of this study was to determine whether ANIT-induced hepatotoxicity and regulation of hepatobiliary transporters are altered in the absence of Nrf2. For this purpose, wild-type and Nrf2-null mice were administered ANIT (75 mg/kg po). Surprisingly, ANIT–induced hepatotoxicity was similar in both genotypes at 48 h. Accumulation of bile acids in serum and liver was lower in Nrf2-null mice compared to wild-types treated with ANIT. Transporter mRNA profiles differed between wild-type and Nrf2-null mice after ANIT. Bsep, Mdr2, and Mrp3 efflux transporters were increased by ANIT in wild-type, but not in Nrf2-null mice. In contrast, mRNA expression of two hepatic uptake transporters, Ntcp and Oatp1b2, were decreased in both genotypes after ANIT, with larger declines in Nrf2-null mice. mRNA expression of the transcriptional repressor of Ntcp, small heterodimeric partner (SHP), was increased in Nrf2-null mice after ANIT. Furthermore, hepatocyte nuclear factor 1{alpha} (HNF1{alpha}), which regulates Oatp1b2, was down-regulated in ANIT-treated Nrf2-null mice. Preferential up-regulation of SHP and down-regulation of HNF1{alpha} and uptake transporters likely explains why Nrf2-null mice exhibited similar injury to wild-types after ANIT. A subsequent study revealed that treatment of mice with the Nrf2 activator oltipraz protects against ANIT-induced histological injury. Despite compensatory changes in Nrf2-null mice to limit ANIT toxicity, pharmacological activation of Nrf2 may represent a therapeutic option for intrahepatic cholestasis.

Key Words: Nrf2; ANIT; Nqo1; oxidative stress; Mrps.


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